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Fusion protein corresponding to Calreticulin. Calreticulin-maltose binding fusion protein.
Our Abpromise guarantee covers the use of ab22683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 63 kDa.|
|IP||Use a concentration of 12.5 µg/ml.|
|Flow Cyt||Use 1-2µg for 106 cells.
(Additional resource: PMID - 18689689)
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Fix and permeabilize cells prior to staining.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ICC/IF image of ab22683 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22683, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Blocked with 5% milk for 1 hour at 23°C.
Incubated with the primary antibody in PBS + 0.5% Tween 20 for 12 hours at 4°C.
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