• Product name
    Anti-Calreticulin antibody [FMC 75] (Phycoerythrin)
    See all Calreticulin primary antibodies
  • Description
    Mouse monoclonal [FMC 75] to Calreticulin (Phycoerythrin)
  • Host species
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Cow, Human, Monkey
  • Immunogen

    Recombinant Calreticulin protein

  • Positive control
    • Jurkat cells.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
  • Purification notes
    Purified from ascites.
  • Clonality
  • Clone number
    FMC 75
  • Isotype
  • Light chain type
  • Research areas


Our Abpromise guarantee covers the use of ab83220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100.

ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.



  • Function
    Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
  • Sequence similarities
    Belongs to the calreticulin family.
  • Domain
    Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
    The interaction with glycans occurs through a binding site in the globular lectin domain.
    The zinc binding sites are localized to the N-domain.
    Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
  • Cellular localization
    Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Autoantigen RO antibody
    • CALR antibody
    • CALR protein antibody
    • CALR_HUMAN antibody
    • Calregulin antibody
    • Calreticulin antibody
    • cC1qR antibody
    • CRP55 antibody
    • CRT antibody
    • CRTC antibody
    • Endoplasmic reticulum resident protein 60 antibody
    • Epididymis secretory sperm binding protein Li 99n antibody
    • ERp60 antibody
    • FLJ26680 antibody
    • grp60 antibody
    • HACBP antibody
    • HEL S 99n antibody
    • RO antibody
    • Sicca syndrome antigen A (autoantigen Ro; calreticulin) antibody
    • Sicca syndrome antigen A antibody
    • SSA antibody
    see all


  • Intracellular staining of 106 Jurkat cells using ab83220 at a concentration of 10µg/ml.


This product has been referenced in:
  • Draganov D  et al. Modulation of P2X4/P2X7/Pannexin-1 sensitivity to extracellular ATP via Ivermectin induces a non-apoptotic and inflammatory form of cancer cell death. Sci Rep 5:16222 (2015). Read more (PubMed: 26552848) »
  • Angelova AL  et al. Complementary induction of immunogenic cell death by oncolytic parvovirus H-1PV and gemcitabine in pancreatic cancer. J Virol 88:5263-76 (2014). Flow Cyt ; Human . Read more (PubMed: 24574398) »
See all 3 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Flow Cytometry
Mouse Cell (B16 melanoma)
Yes - BD Perm/Fix kit was used for intracellular staining
Gating Strategy
Greater than 90% of cells were calreticulin+ compared to PE Isotype control
B16 melanoma
Cell harvesting/tissue preparation method: Cells were trypinised to obtain a single cell suspension.
Sample buffer: Perm/Fix Buffer

Felicia Cao

Verified customer

Submitted Jul 27 2015


According to http://www.uniprot.org/uniprot/P27797 Calreticulin is localized to the ER, cytoplasm, secreted, and on the cell surface, so rather than being a multipass membrane protein it is translocated to different regions of the cell. Thus, uniprot does not list specific amino acid ranges of the protein that are intracellular vs. extracellular when it is localized on the cell surface, and since it is most prevalent in the ER and cytoplasm our anti-Calreticulin antibodies were validated in ICC and flow with fixed, permeabilized cells. Thus, I am not sure whether or not our anti-Calreticulin antibodies, including ab22683, will bind to Calreticulin on the cell surface because I do not know what portion of the protein is extracellular vs. intracellular when it is surface-localized.

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The epitope that this calreticulin antibody recognizes has not been mapped but it is expected that the epitope is on an extracellular region. The unconjugated version of this clone, ab22863, was used for staining live cells for flow cytometry in PubMed: 18689689, http://www.pnas.org/content/105/33/11784.long.

It is not clear how much of the protein is extracellular but it is expected to include the N terminus, based on studies of cell-surface CRT. The review FASEB J. 2010 Mar;24(3):665-83. at http://www.fasebj.org/content/24/3/665.long discusses these studies.

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The immunogen for ab83220 is the full length recombinant protein.

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Vielen Dank für Ihren Anruf.

Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten. Wie am Telefon besprochen habe ich eine kostenlose Ersatzlieferung mit einer anderen Lot# in Auftrag gegeben. Sie hat die Referenznummer 123456 und sollte Montag bei Ihnen ankommen.

Als Anhang finden Sie die beiden Fragebögen für die Flow -Analyse der beiden Antikörper.

Ich wünsche Ihnen viel Erfolg für Ihr Projekt. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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Thank you for your enquiry regarding ab22683.

We have removed Hamster from the "does not react" species list, this seems to be an error.

We apologize for any inconvenience caused and thank you for bringing this matter to our attention.

If you need any further assistance in the future, please do not hesitate to contact me.

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