Anti-Calretinin antibody [1F5H1] (ab204990)
Key features and details
- Mouse monoclonal [1F5H1] to Calretinin
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Calretinin antibody [1F5H1]
See all Calretinin primary antibodies -
Description
Mouse monoclonal [1F5H1] to Calretinin -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow -
Immunogen
Recombinant full length protein corresponding to Human Calretinin aa 1 to the C-terminus. (Purified from E.coli).
Database link: P22676 -
Positive control
- Human Calretinin (aa1-271) recombinant protein; Calretinin (aa1-271)-hIgGFc transfected HEK293 cell lysate; A549 cells; Human brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
1F5H1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204990 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
1/200 - 1/400.
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IHC-P |
1/200 - 1/1000.
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WB |
1/500 - 1/2000. Predicted molecular weight: 31 kDa.
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Notes |
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Flow Cyt
1/200 - 1/400. |
IHC-P
1/200 - 1/1000. |
WB
1/500 - 1/2000. Predicted molecular weight: 31 kDa. |
Target
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Function
Calretinin is a calcium-binding protein which is abundant in auditory neurons. -
Tissue specificity
Brain. -
Sequence similarities
Belongs to the calbindin family.
Contains 6 EF-hand domains. - Information by UniProt
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Database links
- Entrez Gene: 396255 Chicken
- Entrez Gene: 513947 Cow
- Entrez Gene: 794 Human
- Entrez Gene: 12308 Mouse
- Entrez Gene: 117059 Rat
- Omim: 114051 Human
- SwissProt: P07090 Chicken
- SwissProt: Q3ZBY3 Cow
see all -
Alternative names
- 29 kDa calbindin antibody
- CAB 29 antibody
- CAB29 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Calretinin with ab204990 at 1/200 dilution with DAB staining.
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Anti-Calretinin antibody [1F5H1] (ab204990) at 1/500 dilution + Human Calretinin (aa1-271) recombinant protein
Predicted band size: 31 kDaExpected MWt is 57.5 kDa.
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All lanes : Anti-Calretinin antibody [1F5H1] (ab204990) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Calretinin (aa1-271)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 31 kDa -
Flow cytometric analysis of A549 cells labeling Calretinin with ab204990 at 1/200 dilution (green), compared to a negative control (red).
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab204990 has not yet been referenced specifically in any publications.