Key features and details
- Mouse monoclonal [6A9] to Calretinin
- Suitable for: WB, IHC
- Reacts with: Mouse, Rat, Cow
- Isotype: IgA
Product nameAnti-Calretinin antibody [6A9]
See all Calretinin primary antibodies
DescriptionMouse monoclonal [6A9] to Calretinin
Tested applicationsSuitable for: WB, IHCmore details
Species reactivityReacts with: Mouse, Rat, Cow
Recombinant full length protein corresponding to Human Calretinin.
Database link: P22676
- WB: Rat brain and spinal cord, mouse brain and spinal cord, cow spinal cord. IHC: Rat hippocampus.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferConstituents: 50% PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
Purification notesPurified from Tissue Culture supernatant.
Our Abpromise guarantee covers the use of ab277631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/5000. Predicted molecular weight: 31 kDa.|
|IHC||1/2000 - 1/5000.
transcardial perfusion-fixed with 4% paraformaldehyde for 24 hours, free floating sections
FunctionCalretinin is a calcium-binding protein which is abundant in auditory neurons.
Sequence similaritiesBelongs to the calbindin family.
Contains 6 EF-hand domains.
- Information by UniProt
- 29 kDa calbindin antibody
- CAB 29 antibody
- CAB29 antibody
Perfusion-fixed, free floating sections of rat hippocampus section stained for Calretinin using ab277631 (green) at 1/2000 dilution, and counterstained with chicken pAb to parvalbumin (red) at 1/1000 dilution in immunohistochemical analysis. The blue is DAPI staining of nuclear DNA. The calretinin and parvalbumin antibodies stain different subsets of GABAergic interneurons.
Lanes 2-6 : Anti-Calretinin antibody [6A9] (ab277631) at 1/2000 dilution
Lane 1 : Molecular weight ladder
Lane 2 : rat brain
Lane 3 : rat spinal cord
Lane 4 : mouse brain
Lane 5 : mouse spinal cord
Lane 6 : cow spinal cord
Predicted band size: 31 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab277631 has not yet been referenced specifically in any publications.