Overview

  • Product name

  • Description

    Rabbit polyclonal to Calretinin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant full length protein corresponding to Mouse Calretinin.
    Database link: Q08331

  • Positive control

    • WB: Human brain lysate. IHC-P: Human mesothelioma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.6
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary antibody incubation for 10 minutes at room temperature.

WB 1/25. Predicted molecular weight: 31 kDa.

Primary antibody incubation for 1 hour at room temperature.

Target

Images

  • Anti-Calretinin antibody (ab227747) at 1/25 dilution + Human brain lysate

    Predicted band size: 31 kDa

  • Formalin-fixed, paraffin-embedded human mesothelioma tissue stained for Calretinin using ab227747 at 1/200 dilution in immunohistochemical analysis.

References

ab227747 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227747.
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