Overview

  • Product name

  • Description

    Rabbit polyclonal to Calretinin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Zebrafish
    Predicted to work with: Chicken, Cat, Dog, Xenopus laevis
  • Immunogen

    Full length protein corresponding to Calretinin.

  • Positive control

    • IHC-P: Mesothelioma. IHC-Fr: Mouse brain, zebrafish retinal tissue and sea cucumber radial nerve cord tissue.
  • General notes

    Contains a proprietary preservative that is not sodium azide or thimerosal.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.09% Sodium azide
    Constituents: PBS, Carrier protein

    Van Gogh Yellow Diluent
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Myeloma

    unknown
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For an ABC system, dilute 1/50-1/100 and incubate for 30-60 minutes at RT.

IHC-Fr 1/100. PubMed: 20504962

Immunohistochemical studies have recently shown calretinin to be useful in distinguishing mesotheliomas from lung adenocarcinomas. However, it is recommended that a panel of antibodies be used in tandem with calretinin. Other antibodies recommended are CK 5/6, E-cadherin, ESA or BerEP4, CEA, EMA, B72.3, Vimentin and CD15. Calretinin does not mark all mesotheliomas (50-70%).

Target

Images

  • Paraffin embedded mesothelioma (human) stained for Calretinin with ab702 (1/100 dilution) in immunohistochemical analysis.

  • ab702 staining Calretinin in mouse brain tissue sections in immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 3% serum for 30 minutes at 27°C. Samples were incubated with primary antibody (1/250) for 20 hours at 4°C. A Cy3®-conjugated donkey anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

  • ab702 staining Calretinin (green) in zebrafish retinal cells by immunohistochemistry (frozen sections). Embryos were first protected by sequential incubation in 15% then 30% sucrose in phosphate-buffered saline supplemented with 0.5% Triton X-100 (PBST) for 12-16 hours at 4°C, then embedded in OCT, stored at –80°C, and sectioned at 16-20 µm using a cryostat. Embryos subjected to whole-mount in situ hybridisation were cleared in serial incubations of glycerol (25, 50, 75 and 95%), the eyes dissected and placed in a drop of glycerol then cover-slipped. Original article: http://dev.biologists.org/content/137/13/2107.long

  • Immunohistochemical analysis of sea cucumber radial nerve cord tissue, staining Calretinin with ab702 at 1/100 dilution.

References

This product has been referenced in:

  • Akgül G  et al. The Role of AMPARs in the Maturation and Integration of Caudal Ganglionic Eminence-Derived Interneurons into Developing Hippocampal Microcircuits. Sci Rep 9:5435 (2019). Read more (PubMed: 30931998) »
  • Sawant OB  et al. The circadian clock gene Bmal1 is required to control the timing of retinal neurogenesis and lamination of Müller glia in the mouse retina. FASEB J N/A:fj201801832RR (2019). Read more (PubMed: 31002540) »
See all 36 Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain(Hippocampus))
Antigen retrieval step
None
Permeabilization
Yes - 0.2% Triton X
Specification
Brain(Hippocampus)
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Mr. J. Y. Seo

Verified customer

Submitted Apr 03 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (lung)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
lung
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

Mrs. EUN JEUNG KIM

Verified customer

Submitted Jan 12 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Fukomys anselli Tissue sections (Retina)
Permeabilization
Yes - Triton X-100 in Blocking Solution
Specification
Retina
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Davidson's Fixative

Abcam user community

Verified customer

Submitted Jul 31 2017

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 27°C
Sample
Mouse Tissue sections (frozen brain section)
Specification
frozen brain section
Permeabilization
No
Fixative
Paraformaldehyde

Mrs. Bettina Hoerz

Verified customer

Submitted Jan 06 2014

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