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    calretinin-antibody-ep1798-ab92341.pdf

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Neuroscience Neurotransmission Calcium Signaling Calcium Binding Proteins
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RecombinantRabMAb

Recombinant Anti-Calretinin antibody [EP1798] (ab92341)

  • Datasheet
  • SDS
Reviews (5)Q&A (1)References (14)

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Immunocytochemistry/ Immunofluorescence - Anti-Calretinin antibody [EP1798] (ab92341)
  • Western blot - Anti-Calretinin antibody [EP1798] (ab92341)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [EP1798] (ab92341)
  • Flow Cytometry (Intracellular) - Anti-Calretinin antibody [EP1798] (ab92341)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [EP1798] (ab92341)
  • Western blot - Anti-Calretinin antibody [EP1798] (ab92341)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [EP1798] (ab92341)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [EP1798] (ab92341)
  • Anti-Calretinin antibody [EP1798] (ab92341)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1798] to Calretinin
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free PE

You may also be interested in

Protein
Product image
Recombinant Human Calretinin protein (ab100941)
Primary
Product image
Anti-Calbindin antibody [EP3478] (ab108404)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-Calretinin antibody [EP1798]
    See all Calretinin primary antibodies
  • Description

    Rabbit monoclonal [EP1798] to Calretinin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse brain, Human brain and Rat brain tissue lysates. Flow Cyt (Intra): SH-SY5Y cells. IHC-P: Mouse cerebrum, Rat cerebrum, Human cerebrum, and Human mesothelioma tissues. ICC/IF: SH-SY5Y cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1798
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • Calcium Binding Proteins
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • PE Anti-Calretinin antibody [EP1798] (ab210633)
    • Alexa Fluor® 647 Anti-Calretinin antibody [EP1798] (ab214244)
    • Anti-Calretinin antibody [EP1798] - BSA and Azide free (ab232462)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Calretinin protein (ab100941)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab92341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For unpurified use at 1/100.

WB
1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
IHC-P (3)
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250.

ICC/IF
1/100 - 1/250.
Notes
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For unpurified use at 1/100.

WB
1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
IHC-P
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250.

ICC/IF
1/100 - 1/250.

Target

  • Function

    Calretinin is a calcium-binding protein which is abundant in auditory neurons.
  • Tissue specificity

    Brain.
  • Sequence similarities

    Belongs to the calbindin family.
    Contains 6 EF-hand domains.
  • Target information above from: UniProt accession P22676 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 794 Human
    • Entrez Gene: 12308 Mouse
    • Entrez Gene: 117059 Rat
    • Omim: 114051 Human
    • SwissProt: P22676 Human
    • SwissProt: Q08331 Mouse
    • SwissProt: P47728 Rat
    • Unigene: 106857 Human
    • Unigene: 2755 Mouse
    • Unigene: 10321 Rat
    see all
  • Alternative names

    • 29 kDa calbindin antibody
    • CAB 29 antibody
    • CAB29 antibody
    • CAL 2 antibody
    • CAL2 antibody
    • CALB 2 antibody
    • CALB2 antibody
    • CALB2_HUMAN antibody
    • Calbindin 2 29kDa antibody
    • Calbindin 2 antibody
    • Calbindin D29K antibody
    • Calbindin2 antibody
    • Calretinin antibody
    • CR antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Calretinin antibody [EP1798] (ab92341)
    Immunocytochemistry/ Immunofluorescence - Anti-Calretinin antibody [EP1798] (ab92341)

    Immunofluorecent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilsed SH-SY5Y (human neuroblastoma epithelial cell line) cells labelling Calretinin with ab92341 at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit (ab150077) secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and nuclear staining on SH-SY5Y cell line is observed. Counterstained with DAPI.

  • Western blot - Anti-Calretinin antibody [EP1798] (ab92341)
    Western blot - Anti-Calretinin antibody [EP1798] (ab92341)
    All lanes : Anti-Calretinin antibody [EP1798] (ab92341) at 1/1000 dilution (Purified)

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [EP1798] (ab92341)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [EP1798] (ab92341)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling Calretinin with purified ab92341 at 1:4000 (0.034 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Flow Cytometry (Intracellular) - Anti-Calretinin antibody [EP1798] (ab92341)
    Flow Cytometry (Intracellular) - Anti-Calretinin antibody [EP1798] (ab92341)
    Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling Calretinin with purified ab92341 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150081) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [EP1798] (ab92341)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [EP1798] (ab92341)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling Calretinin with purified ab92341 at 1:4000 (0.034 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-Calretinin antibody [EP1798] (ab92341)
    Western blot - Anti-Calretinin antibody [EP1798] (ab92341)
    Anti-Calretinin antibody [EP1798] (ab92341) at 1/1000 dilution (Purified) + Human brain lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [EP1798] (ab92341)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [EP1798] (ab92341)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Calretinin with purified ab92341 at 1:4000 (0.034 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [EP1798] (ab92341)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [EP1798] (ab92341)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human mesothelioma tissue sections labeling Calretinin with purified ab92341 at 1:4000 (0.034 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Anti-Calretinin antibody [EP1798] (ab92341)
    Anti-Calretinin antibody [EP1798] (ab92341)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (14)

Publishing research using ab92341? Please let us know so that we can cite the reference in this datasheet.

ab92341 has been referenced in 14 publications.

  • Cai J  et al. Exosomes in ovarian cancer ascites promote epithelial-mesenchymal transition of ovarian cancer cells by delivery of miR-6780b-5p. Cell Death Dis 12:210 (2021). PubMed: 33627627
  • Strippoli R  et al. Caveolin1 and YAP drive mechanically induced mesothelial to mesenchymal transition and fibrosis. Cell Death Dis 11:647 (2020). PubMed: 32811813
  • Deryckere A  et al. Multifaceted actions of Zeb2 in postnatal neurogenesis from the ventricular-subventricular zone to the olfactory bulb. Development 147:N/A (2020). PubMed: 32253238
  • Duan L  et al. HVEM/HIF-1a promoted proliferation and inhibited apoptosis of ovarian cancer cells under hypoxic microenvironment conditions. J Ovarian Res 13:40 (2020). PubMed: 32312328
  • Trujillo CA  et al. Complex Oscillatory Waves Emerging from Cortical Organoids Model Early Human Brain Network Development. Cell Stem Cell 25:558-569.e7 (2019). PubMed: 31474560
View all Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Calretinin antibody [EP1798]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: buffer pH6
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Read More

DR. Didier Pin

Verified customer

Submitted Nov 09 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Calretinin antibody [EP1798]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain (cortex))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Specification
brain (cortex)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Nov 02 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Calretinin antibody [EP1798]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain (thalamus))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Specification
brain (thalamus)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Nov 02 2020

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