RecombinantRabMAb

Recombinant Anti-Calretinin antibody [EP1798] (ab92341)

Reviews (4)Q&A (1)References (8)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1798] to Calretinin
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Calretinin antibody [EP1798]
    See all Calretinin primary antibodies

  • Description

    Rabbit monoclonal [EP1798] to Calretinin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Calretinin aa 50-150. The exact sequence is proprietary.
    Database link: P22676

  • Positive control

    • Human fetal, Mouse and Rat brain lysates; Human brain and mesothelioma tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EP1798

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab92341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.

    • Target

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [EP1798] (ab92341)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [EP1798] (ab92341)

      ab92341 at 1/100 dilution staining Calretinin in Human mesothelioma by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-Calretinin antibody [EP1798] (ab92341)
      Western blot - Anti-Calretinin antibody [EP1798] (ab92341)
      All lanes : Anti-Calretinin antibody [EP1798] (ab92341) at 1/1000 dilution

      Lane 1 : Human fetal Brain lysate
      Lane 2 : Mouse brain lysate
      Lane 3 : Rat brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 29 kDa
      Observed band size: 29 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [EP1798] (ab92341)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [EP1798] (ab92341)

      ab92341 at 1/100 dilution staining Calretinin in Human brain by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-Calretinin antibody [EP1798] (ab92341)
      Flow Cytometry - Anti-Calretinin antibody [EP1798] (ab92341)
      Overlay histogram showing SH-SY5Y cells stained with ab92341 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92341, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Immunocytochemistry/ Immunofluorescence - Anti-Calretinin antibody [EP1798] (ab92341)
      Immunocytochemistry/ Immunofluorescence - Anti-Calretinin antibody [EP1798] (ab92341)

      Immunofluorecent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilsed SH-SY5Y (human neuroblastoma epithelial cell line) cells labelling Calretinin with ab92341 at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit (ab150077) secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and nuclear staining on SH-SY5Y cell line is observed. Counterstained with DAPI.

    • Anti-Calretinin antibody [EP1798] (ab92341)
      Anti-Calretinin antibody [EP1798] (ab92341)

    References (8)

    Publishing research using ab92341? Please let us know so that we can cite the reference in this datasheet.

    ab92341 has been referenced in 8 publications.

    • Duan L  et al. HVEM/HIF-1a promoted proliferation and inhibited apoptosis of ovarian cancer cells under hypoxic microenvironment conditions. J Ovarian Res 13:40 (2020). PubMed: 32312328
    • Bovio PP  et al. Differential Methylation of H3K79 Reveals DOT1L Target Genes and Function in the Cerebellum In Vivo. Mol Neurobiol 56:4273-4287 (2019). PubMed: 30302725
    • Kawka E  et al. EPITHELIAL-TO-MESENCHYMAL TRANSITION AND MIGRATION OF HUMAN PERITONEAL MESOTHELIAL CELLS UNDERGOING SENESCENCE. Perit Dial Int N/A:N/A (2018). PubMed: 30478141
    • Francavilla C  et al. Phosphoproteomics of Primary Cells Reveals Druggable Kinase Signatures in Ovarian Cancer. Cell Rep 18:3242-3256 (2017). ICC/IF ; Human . PubMed: 28355574
    • McMahon SM  et al. Multiple cytosolic calcium buffers in posterior pituitary nerve terminals. J Gen Physiol 147:243-54 (2016). WB ; Rat . PubMed: 26880753
    • Takagi T  et al. De novo inbred heterozygous Zeb2/Sip1 mutant mice uniquely generated by germ-line conditional knockout exhibit craniofacial, callosal and behavioral defects associated with Mowat-Wilson syndrome. Hum Mol Genet 24:6390-402 (2015). IHC-P ; Mouse . PubMed: 26319231
    • Tokunaga CC  et al. Effects of anti-VEGF treatment on the recovery of the developing retina following oxygen-induced retinopathy. Invest Ophthalmol Vis Sci 55:1884-92 (2014). IHC ; Mouse . PubMed: 24550366
    • Endesfelder S  et al. Caffeine protects neuronal cells against injury caused by hyperoxia in the immature brain. Free Radic Biol Med 67C:221-234 (2013). Rat . PubMed: 24129198

    Customer reviews and Q&As

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    1-5 of 5 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Calretinin antibody [EP1798]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (brain (cortex))
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
    Permeabilization
    No
    Specification
    brain (cortex)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 02 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Calretinin antibody [EP1798]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain (thalamus))
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
    Permeabilization
    No
    Specification
    brain (thalamus)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 02 2020

    Immunohistochemistry free floating abreview for Anti-Calretinin antibody [EP1798]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (brainstem)
    Specification
    brainstem
    Read More

    Lin Lin

    Verified customer

    Submitted Jun 16 2017

    Immunohistochemistry (Frozen sections)

     Below Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Permeabilization
    Yes - 0.1% Triton-X (in PBS)
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 12 2013

    Question

    I would ask an information about your primary antibodies: anti-Calretinin EPR 1799(2) cod. ab 133316 and anti-Calretinin EP 1798 cod. ab 92341. The SH-SY5Y cells, used as positive controls in Western Blotting and immuonfluorescence, are undifferentiated or differentiated cells?

    Read More
    Answer


    The SH-SY5Y cell line is a thrice-cloned sub-line of bone marrow biopsy-derived line SK-N-SH. SH-SY-5Y has dopamine-β-hydroxylase activity and can convert glutamate to the neurotransmitter GABA.

    Read More

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