Recombinant Anti-Calretinin antibody [EP1798] (ab92341)

Immunofluorecent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilsed SH-SY5Y (human neuroblastoma epithelial cell line) cells labelling Calretinin with ab92341 at 1/500 dilution, followed by AlexaFluor^®488 Goat anti-Rabbit (ab150077) secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and nuclear staining on SH-SY5Y cell line is observed. Counterstained with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling Calretinin with purified ab92341 at 1:4000 (0.034 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling Calretinin with purified ab92341 at 1:4000 (0.034 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Calretinin with purified ab92341 at 1:4000 (0.034 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human mesothelioma tissue sections labeling Calretinin with purified ab92341 at 1:4000 (0.034 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.