Product nameAnti-Calretinin antibody [EPR1799(2)]
See all Calretinin primary antibodies
DescriptionRabbit monoclonal [EPR1799(2)] to Calretinin
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues in Human Calretinin (P22676).
- Fetal brain, SH-SY5Y, 293T, fetal kidney, and U87-MG lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 31 kDa.|
|IP||1/10 - 1/100.|
|ICC/IF||1/50 - 1/100.|
FunctionCalretinin is a calcium-binding protein which is abundant in auditory neurons.
Sequence similaritiesBelongs to the calbindin family.
Contains 6 EF-hand domains.
- Information by UniProt
- 29 kDa calbindin antibody
- CAB 29 antibody
- CAB29 antibody
ab133316 staining Calretinin in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. ab150081, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
All lanes : Anti-Calretinin antibody [EPR1799(2)] (ab133316) at 1/1000 dilution
Lane 1 : Fetal brain lysates
Lane 2 : SH-SY5Y lysates
Lane 3 : 293T lysates
Lane 4 : Fetal kidney lysates
Lane 5 : U87-MG lysates
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 31 kDa
ab133316 has not yet been referenced specifically in any publications.