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RecombinantRabMAb

Recombinant Anti-Calretinin antibody [EPR1799(2)] (ab133316)

  • Datasheet
Reviews (2)Q&A (1)

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Western blot - Anti-Calretinin antibody [EPR1799(2)] (ab133316)
  • Anti-Calretinin antibody [EPR1799(2)] (ab133316)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1799(2)] to Calretinin
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Protein
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Recombinant Human Calretinin protein (ab100941)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-Calretinin antibody [EPR1799(2)]
    See all Calretinin primary antibodies
  • Description

    Rabbit monoclonal [EPR1799(2)] to Calretinin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Calretinin (P22676).

  • Positive control

    • Fetal brain, SH-SY5Y, 293T, fetal kidney, and U87-MG lysates
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR1799(2)
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • Calcium Binding Proteins

Associated products

  • Alternative Versions

    • Anti-Calretinin antibody [EPR1799(2)] - BSA and Azide free (ab248469)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Calretinin protein (ab100941)

Applications

Our Abpromise guarantee covers the use of ab133316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function

      Calretinin is a calcium-binding protein which is abundant in auditory neurons.
    • Tissue specificity

      Brain.
    • Sequence similarities

      Belongs to the calbindin family.
      Contains 6 EF-hand domains.
    • Target information above from: UniProt accession P22676 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 794 Human
      • Entrez Gene: 12308 Mouse
      • Entrez Gene: 117059 Rat
      • Omim: 114051 Human
      • SwissProt: P22676 Human
      • SwissProt: Q08331 Mouse
      • SwissProt: P47728 Rat
      • Unigene: 106857 Human
      • Unigene: 2755 Mouse
      • Unigene: 10321 Rat
      see all
    • Alternative names

      • 29 kDa calbindin antibody
      • CAB 29 antibody
      • CAB29 antibody
      • CAL 2 antibody
      • CAL2 antibody
      • CALB 2 antibody
      • CALB2 antibody
      • CALB2_HUMAN antibody
      • Calbindin 2 29kDa antibody
      • Calbindin 2 antibody
      • Calbindin D29K antibody
      • Calbindin2 antibody
      • Calretinin antibody
      • CR antibody
      see all

    Images

    • Western blot - Anti-Calretinin antibody [EPR1799(2)] (ab133316)
      Western blot - Anti-Calretinin antibody [EPR1799(2)] (ab133316)
      All lanes : Anti-Calretinin antibody [EPR1799(2)] (ab133316) at 1/1000 dilution

      Lane 1 : Fetal brain lysates
      Lane 2 : SH-SY5Y lysates
      Lane 3 : 293T lysates
      Lane 4 : Fetal kidney lysates
      Lane 5 : U87-MG lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 31 kDa

    • Anti-Calretinin antibody [EPR1799(2)] (ab133316)
      Anti-Calretinin antibody [EPR1799(2)] (ab133316)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab133316? Please let us know so that we can cite the reference in this datasheet.

    ab133316 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews

    Immunohistochemistry free floating abreview for Anti-Calretinin antibody [EPR1799(2)]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Neuron)
    Specification
    Neuron
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 31 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Calretinin [EPR1799(2)] antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    Yes - 0.5% Triton-X100 in PBS
    Fixative
    Paraformaldehyde
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Jul 18 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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