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I would ask an information about your primary antibodies: anti-Calretinin EPR 1799(2) cod. ab 133316 and anti-Calretinin EP 1798 cod. ab 92341. The SH-SY5Y cells, used as positive controls in Western Blotting and immuonfluorescence, are undifferentiated or differentiated cells?
Asked on Dec 22 2012