The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
We suggest an incubation period of 30 minutes at room temperature or overnight at 4 degrees Celsius. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Calretinin is a calcium-binding protein which is abundant in auditory neurons.
Belongs to the calbindin family. Contains 6 EF-hand domains.