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Recombinant full length protein within Mouse Calretinin. The exact sequence is proprietary.
Our Abpromise guarantee covers the use of ab16694 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Immunocytochemical analysis of 5% PFA & 0.2% Picric Acid fixed rat cortical cell sections, labelling Calretinin with ab16694 at a dilution of 1/10 incubated fro 12 hours at 4°C in a 10mM PBS & 0.35% Triton X diluent. Secondary was a donkey anti-rabbit polyclonal Alexa Fluor® 488 conjugate at 1/200.
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