Overview

  • Product name
    Anti-Calretinin antibody [SP13]
    See all Calretinin primary antibodies
  • Description
    Rabbit monoclonal [SP13] to Calretinin
  • Host species
    Rabbit
  • Specificity
    Ab16694 recognises Calretinin.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein within Mouse Calretinin. The exact sequence is proprietary.

  • Positive control
    • Human mesothelioma for Immunohistochemistry. IHC-Fr: Mouse and rat cerebrum tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.5
    Preservative: 0.1% Sodium azide
    Constituents: Tissue culture supernatant, Tris buffered saline, 1% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    SP13
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/200.
IHC-Fr 1/30.

Target

Images

  • Immunohistochemistry (Frozen) analysis of mouse cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunocytochemical analysis of 5% PFA & 0.2% Picric Acid fixed rat cortical cell sections, labelling Calretinin with ab16694 at a dilution of 1/10 incubated fro 12 hours at 4°C in a 10mM PBS & 0.35% Triton X diluent. Secondary was a donkey anti-rabbit polyclonal Alexa Fluor® 488 conjugate at 1/200.

    See Abreview

  • Immunohistochemistry (Frozen) analysis of rat cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Frozen) analysis of rat cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Frozen) analysis of mouse cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Ab16694 at a dilution of 1/100, staining Calretinin in formalin fixed paraffin embedded human mesothelioma tissue section by Immunohistochemistry.

References

This product has been referenced in:
  • Ohara Y  et al. Connective tissue growth factor-specific monoclonal antibody inhibits growth of malignant mesothelioma in an orthotopic mouse model. Oncotarget 9:18494-18509 (2018). Read more (PubMed: 29719620) »
  • Yin M  et al. Gremlin-1 is a key regulator of the invasive cell phenotype in mesothelioma. Oncotarget 8:98280-98297 (2017). Read more (PubMed: 29228689) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Asytanax mexicanus Tissue sections (Adult fish skull)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate Buffer
Permeabilization
No
Specification
Adult fish skull
Blocking step
We use the protien block in the ab64261- Rabbit specific HRP/DAB (ABC) Detection kit as blocking agent for 10 minute(s) · Concentration: 10µg/mL · Temperature: 24°C
Fixative
Paraformaldehyde

Dr. Devi Atukorallaya

Verified customer

Submitted Aug 06 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat Cortical Cell Culture)
Permeabilization
No
Specification
Rat Cortical Cell Culture
Fixative
5% PF, 02% Picric Acid

Ms. Babben Tinner

Verified customer

Submitted Oct 07 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up