RabMAb

Anti-Calretinin antibody [SP13] - BSA and Azide free (ab239795)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [SP13] - BSA and Azide free (ab239795)
  • Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] - BSA and Azide free (ab239795)
  • Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] - BSA and Azide free (ab239795)
  • Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] - BSA and Azide free (ab239795)
  • Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] - BSA and Azide free (ab239795)

Key features and details

  • Rabbit monoclonal [SP13] to Calretinin - BSA and Azide free
  • Suitable for: IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Calretinin antibody [SP13] - BSA and Azide free
    See all Calretinin primary antibodies

  • Description

    Rabbit monoclonal [SP13] to Calretinin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant full length protein within Mouse Calretinin. The exact sequence is proprietary.

  • Positive control

    • IHC-P: Human mesothelioma tissue.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Ab239795 is the carrier-free version of ab16694. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239795 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab239795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [SP13] - BSA and Azide free (ab239795)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [SP13] - BSA and Azide free (ab239795)

    ab16694 at a dilution of 1/100, staining Calretinin in formalin fixed paraffin embedded human mesothelioma tissue section by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing Tris buffered saline, BSA, and sodium azide (ab16694).

  • Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] - BSA and Azide free (ab239795)
    Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] - BSA and Azide free (ab239795)

    Immunohistochemistry (Frozen) analysis of rat cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab16694).

  • Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] - BSA and Azide free (ab239795)
    Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] - BSA and Azide free (ab239795)

    Immunohistochemistry (Frozen) analysis of rat cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab16694).

  • Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] - BSA and Azide free (ab239795)
    Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] - BSA and Azide free (ab239795)

    Immunohistochemistry (Frozen) analysis of mouse cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab16694).

  • Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] - BSA and Azide free (ab239795)
    Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] - BSA and Azide free (ab239795)

    Immunohistochemistry (Frozen) analysis of mouse cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab16694).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab239795? Please let us know so that we can cite the reference in this datasheet.

ab239795 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com