Calretinin ELISA Kit (ab233616)

Calretinin in vitro SimpleStep ELISA^® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Calretinin protein in human, mouse, and ratbrain extract, and breast milk.

The SimpleStep ELISA^® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

Calretinin, also known as 29 kDa calbindin, is a calcium-binding member of the troponin C superfamily and is encoded by the CALB2 gene. Calretinin is a 271- amino acid protein that contains six Ca²⁺ chelating EF-hand domains, and is highly conserved across several species e.g., mouse and rat. Calretinin shares 58.7% amino acid sequence identity of human calbindin, which is in the same family of calcium binding proteins. Calretinin is highly expressed in neurons including the retina, and is involved in intracellular calcium buffering, message targeting, and modulates neuronal excitability. Calretinin serves as a diagnostic marker for some human diseases, such as Hirschsprung disease, as well as used to distinguish between neuronal and glial types of brain tumors.  

• Neuroscience
• Neurotransmission
• Calcium Signaling
• Calcium Binding Proteins