Overview

  • Product name

    Calretinin ELISA Kit
    See all Calretinin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Brain 6 4.5%
    Inter-assay
    Sample n Mean SD CV%
    Brain 3 10.5%
  • Sample type

    Milk, Tissue Extracts, Mouse brain extract
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    4.2 pg/ml
  • Range

    234 pg/ml - 15000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Milk 111 103% - 115%
    Tissue Extracts 107 98% - 114%
    Mouse brain extract 106 97% - 115%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Product overview

    Calretinin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Calretinin protein in human, mouse, and ratbrain extract, and breast milk.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Calretinin, also known as 29 kDa calbindin, is a calcium-binding member of the troponin C superfamily and is encoded by the CALB2 gene. Calretinin is a 271- amino acid protein that contains six Ca2+ chelating EF-hand domains, and is highly conserved across several species e.g., mouse and rat. Calretinin shares 58.7% amino acid sequence identity of human calbindin, which is in the same family of calcium binding proteins. Calretinin is highly expressed in neurons including the retina, and is involved in intracellular calcium buffering, message targeting, and modulates neuronal excitability. Calretinin serves as a diagnostic marker for some human diseases, such as Hirschsprung disease, as well as used to distinguish between neuronal and glial types of brain tumors.  

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab233616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • The Calretinin standard curve was prepared as described in Section 10.

  • The concentrations of Calretinin were measured in duplicates, interpolated from the Calretinin standard curves and corrected for sample dilution. Undiluted samples are as follows: brain extract 12.5 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • The concentrations of Calretinin were measured in duplicates, interpolated from the Calretinin standard curves and corrected for sample dilution. Undiluted samples are as follows: milk 1.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Calretinin concentration was determined to be 227 ng/mL in neat human breast milk.

  • Calretinin expression in non-neural and non-malignant tissues are expected to be significantly lower than neural tissues. Interpolated concentrations of native Calretinin in human brain, heart, liver, and placenta extract samples. The concentrations of Calretinin were measured in duplicates, interpolated from the Calretinin standard curves and corrected for sample dilution. Undiluted samples are as follows: brain extract 3 µg/mL, heart, liver, and placenta extract 100 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Protocols

References

ab233616 has not yet been referenced specifically in any publications.

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