For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    calretinin-elisa-kit-ab233616.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Neurotransmission Calcium Signaling Calcium Binding Proteins
Share by email
RecombinantSimpleStep

Calretinin ELISA Kit (ab233616)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Other - Calretinin ELISA Kit (ab233616)
  • Example of Calretinin standard curve in 1X Cell Extraction Buffer PTR.
  • Interpolated concentrations of native Calretinin in human, mouse, and rat brain extract samples.
  • Interpolated concentrations of native Calretinin in human breast milk samples.
  • Relative expression of calretinin in normal tissue extracts
  • Sandwich ELISA - Calretinin ELISA Kit (ab233616)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 4.2 pg/ml
  • Range: 234 pg/ml - 15000 pg/ml
  • Sample type: Milk, Mouse brain extract, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Assay
Product image
Fura-2 Calcium Flux Assay Kit - No Wash, Ratiometric (ab176766)
ELISA
Product image
S100B ELISA Kit (ab234573)
Pair
Product image
Calretinin Antibody Pair - BSA and Azide free (ab244089)

View more associated products

Overview

  • Product name

    Calretinin ELISA Kit
    See all Calretinin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Brain 6 4.5%
    Inter-assay
    Sample n Mean SD CV%
    Brain 3 10.5%
  • Sample type

    Milk, Tissue Extracts, Mouse brain extract
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    4.2 pg/ml
  • Range

    234 pg/ml - 15000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Milk 111 103% - 115%
    Tissue Extracts 107 98% - 114%
    Mouse brain extract 106 97% - 115%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Product overview

    Calretinin ELISA Kit (ab233616) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Calretinin protein in milk, mouse brain extract, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Calretinin with 4.2 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Calretinin Capture Antibody 1 x 600µl
    10X Calretinin Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 5BR 1 x 6ml
    Calretinin Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • Calcium Binding Proteins
    • Neuroscience
    • Processes
  • Function

    Calretinin is a calcium-binding protein which is abundant in auditory neurons.
  • Tissue specificity

    Brain.
  • Sequence similarities

    Belongs to the calbindin family.
    Contains 6 EF-hand domains.
  • Target information above from: UniProt accession P22676 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • 29 kDa calbindin
    • CAB 29
    • CAB29
    • CAL 2
    • CAL2
    • CALB 2
    • CALB2
    • CALB2_HUMAN
    • Calbindin 2
    • Calbindin 2 29kDa
    • Calbindin D29K
    • Calbindin2
    • Calretinin
    • CR
    see all
  • Database links

    • Entrez Gene: 794 Human
    • Entrez Gene: 12308 Mouse
    • Entrez Gene: 117059 Rat
    • Omim: 114051 Human
    • SwissProt: P22676 Human
    • SwissProt: Q08331 Mouse
    • SwissProt: P47728 Rat
    • Unigene: 106857 Human
    • Unigene: 2755 Mouse
    • Unigene: 10321 Rat
    see all

Associated products

  • Alternative Versions

    • Calretinin Antibody Pair - BSA and Azide free (ab244089)

Images

  • Other - Calretinin ELISA Kit (ab233616)
    Other - Calretinin ELISA Kit (ab233616)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of Calretinin standard curve in 1X Cell Extraction Buffer PTR.
    Example of Calretinin standard curve in 1X Cell Extraction Buffer PTR.

    The Calretinin standard curve was prepared as described in Section 10.

  • Interpolated concentrations of native Calretinin in human, mouse, and rat brain extract samples.
    Interpolated concentrations of native Calretinin in human, mouse, and rat brain extract samples.

    The concentrations of Calretinin were measured in duplicates, interpolated from the Calretinin standard curves and corrected for sample dilution. Undiluted samples are as follows: brain extract 12.5 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Interpolated concentrations of native Calretinin in human breast milk samples.
    Interpolated concentrations of native Calretinin in human breast milk samples.

    The concentrations of Calretinin were measured in duplicates, interpolated from the Calretinin standard curves and corrected for sample dilution. Undiluted samples are as follows: milk 1.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Calretinin concentration was determined to be 227 ng/mL in neat human breast milk.

  • Relative expression of calretinin in normal tissue extracts
    Relative expression of calretinin in normal tissue extracts

    Calretinin expression in non-neural and non-malignant tissues are expected to be significantly lower than neural tissues. Interpolated concentrations of native Calretinin in human brain, heart, liver, and placenta extract samples. The concentrations of Calretinin were measured in duplicates, interpolated from the Calretinin standard curves and corrected for sample dilution. Undiluted samples are as follows: brain extract 3 µg/mL, heart, liver, and placenta extract 100 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Sandwich ELISA - Calretinin ELISA Kit (ab233616)
    Sandwich ELISA - Calretinin ELISA Kit (ab233616)
    To learn more about the advantages of recombinant antibodies see here.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab233616? Please let us know so that we can cite the reference in this datasheet.

ab233616 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab233616.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.