Key features and details
- Rabbit polyclonal to Calsequestrin 2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Calsequestrin 2 antibody
See all Calsequestrin 2 primary antibodies
DescriptionRabbit polyclonal to Calsequestrin 2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Cow
Recombinant fragment corresponding to Human Calsequestrin 2 aa 124-398.
Database link: O14958
- IHC-P: Cal27 xenograft, mouse muscle tissue. WB: H1299 whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96387 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000. Antigen retrieval is not essential but may optimise staining.|
|WB||1/500 - 1/3000. Predicted molecular weight: 46 kDa.|
FunctionCalsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. The release of calcium bound to calsequestrin through a calcium release channel triggers muscle contraction. Binds 40 to 50 moles of calcium.
Involvement in diseaseDefects in CASQ2 are the cause of catecholaminergic polymorphic ventricular tachycardia type 2 (CPVT2) [MIM:611938]; also known as stress-induced polymorphic ventricular tachycardia (VTSIP). CPVT2 is an autosomal recessive form of arrhythmogenic disorder characterized by stress-induced, bidirectional ventricular tachycardia that may degenerate into cardiac arrest and cause sudden death.
Sequence similaritiesBelongs to the calsequestrin family.
Cellular localizationSarcoplasmic reticulum lumen. This isoform of calsequestrin occurs in the sarcoplasmic reticulum's terminal cisternae luminal spaces of cardiac and slow skeletal muscle cells.
- Information by UniProt
- AA033488 antibody
- AW146219 antibody
- Calsequestrin 2 (cardiac muscle) antibody
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ab96387 at 1:500 dilution.
Anti-Calsequestrin 2 antibody (ab96387) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 46 kDa
10% SDS PAGE
Paraffin embedded mouse muscle tissue stained for Calsequestrin-2 using ab96387 at 1/500 dilution in immunohistochemical analysis.
ab96387 has not yet been referenced specifically in any publications.