Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4227] to Calsequestrin 2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-Calsequestrin 2 antibody [EPR4227]
See all Calsequestrin 2 primary antibodies
DescriptionRabbit monoclonal [EPR4227] to Calsequestrin 2
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Calsequestrin 2 aa 1-100. The exact sequence is proprietary.
- Human heart, mouse heart or rat heart lysates
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab108289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 46 kDa).|
FunctionCalsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. The release of calcium bound to calsequestrin through a calcium release channel triggers muscle contraction. Binds 40 to 50 moles of calcium.
Involvement in diseaseDefects in CASQ2 are the cause of catecholaminergic polymorphic ventricular tachycardia type 2 (CPVT2) [MIM:611938]; also known as stress-induced polymorphic ventricular tachycardia (VTSIP). CPVT2 is an autosomal recessive form of arrhythmogenic disorder characterized by stress-induced, bidirectional ventricular tachycardia that may degenerate into cardiac arrest and cause sudden death.
Sequence similaritiesBelongs to the calsequestrin family.
Cellular localizationSarcoplasmic reticulum lumen. This isoform of calsequestrin occurs in the sarcoplasmic reticulum's terminal cisternae luminal spaces of cardiac and slow skeletal muscle cells.
- Information by UniProt
- AA033488 antibody
- AW146219 antibody
- Calsequestrin 2 (cardiac muscle) antibody
All lanes : Anti-Calsequestrin 2 antibody [EPR4227] (ab108289) at 1/1000 dilution
Lane 1 : Human heart cell lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat heart lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 46 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
ab108289 has been referenced in 5 publications.
- Kirsh AJ et al. Dietary nitrate does not alter cardiac function, calcium handling proteins, or SERCA activity in the left ventricle of healthy rats. Appl Physiol Nutr Metab N/A:N/A (2020). PubMed: 32379978
- Jannas-Vela S et al. Resting metabolic rate and skeletal muscle SERCA and Na+ /K+ ATPase activities are not affected by fish oil supplementation in healthy older adults. Physiol Rep 8:e14408 (2020). PubMed: 32342642
- Geng L et al. Rapid Electrical Stimulation Increased Cardiac Apoptosis Through Disturbance of Calcium Homeostasis and Mitochondrial Dysfunction in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Cell Physiol Biochem 47:1167-1180 (2018). PubMed: 29913448
- Estrada-Avilés R et al. The cardiac calsequestrin gene transcription is modulated at the promoter by NFAT and MEF-2 transcription factors. PLoS One 12:e0184724 (2017). PubMed: 28886186
- Curran J et al. EHD3-dependent endosome pathway regulates cardiac membrane excitability and physiology. Circ Res 115:68-78 (2014). PubMed: 24759929