Anti-Calumenin antibody (ab241316)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Calumenin antibody
See all Calumenin primary antibodies -
Description
Rabbit polyclonal to Calumenin -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan
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Immunogen
Synthetic peptide within Human Calumenin aa 200-250. The exact sequence is proprietary.
Database link: O43852 -
Positive control
- IP: HEK-293T whole cell lysate.
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General notes
ab241316 has not performed satisfactorily when used for WB of Calumenin in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Calumenin.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab241316 was affinity purified using an epitope specific to Calumenin immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab241316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Involved in regulation of vitamin K-dependent carboxylation of multiple amino-terminal glutamate residues. Seems to inhibit gamma-carboxylase GGCX. Binds 7 calcium ions with a low affinity. -
Tissue specificity
Ubiquitously expressed. Expressed at high levels in heart, placenta and skeletal muscle, at lower levels in lung, kidney and pancreas and at very low levels in brain and liver. -
Sequence similarities
Belongs to the CREC family.
Contains 6 EF-hand domains. -
Cellular localization
Endoplasmic reticulum lumen. Secreted. Melanosome. Sarcoplasmic reticulum lumen. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 813 Human
- Entrez Gene: 100171969 Orangutan
- Omim: 603420 Human
- SwissProt: O43852 Human
- Unigene: 592258 Human
- Unigene: 7753 Human
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Alternative names
- CALU antibody
- CALU_HUMAN antibody
- Calumenin 1 antibody
see all
Images
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Immunoprecipitation - Anti-Calumenin antibody (ab241316)Calumenin was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241316 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241316 at 1 µg/ml.
Lane 1: ab241316 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Lysate prepared using NETN lysis buffer.
Datasheets and documents
References
ab241316 has not yet been referenced specifically in any publications.
