Anti-Calumenin antibody (ab241316)
Key features and details
- Rabbit polyclonal to Calumenin
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Calumenin antibody
See all Calumenin primary antibodies -
Description
Rabbit polyclonal to Calumenin -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan
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Immunogen
Synthetic peptide within Human Calumenin aa 200-250. The exact sequence is proprietary.
Database link: O43852 -
Positive control
- IP: HEK-293T whole cell lysate.
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General notes
ab241316 has not performed satisfactorily when used for WB of Calumenin in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Calumenin.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab241316 was affinity purified using an epitope specific to Calumenin immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab241316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Involved in regulation of vitamin K-dependent carboxylation of multiple amino-terminal glutamate residues. Seems to inhibit gamma-carboxylase GGCX. Binds 7 calcium ions with a low affinity. -
Tissue specificity
Ubiquitously expressed. Expressed at high levels in heart, placenta and skeletal muscle, at lower levels in lung, kidney and pancreas and at very low levels in brain and liver. -
Sequence similarities
Belongs to the CREC family.
Contains 6 EF-hand domains. -
Cellular localization
Endoplasmic reticulum lumen. Secreted. Melanosome. Sarcoplasmic reticulum lumen. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 813 Human
- Entrez Gene: 100171969 Orangutan
- Omim: 603420 Human
- SwissProt: O43852 Human
- Unigene: 592258 Human
- Unigene: 7753 Human
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Alternative names
- CALU antibody
- CALU_HUMAN antibody
- Calumenin 1 antibody
see all
Images
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Immunoprecipitation - Anti-Calumenin antibody (ab241316)
Calumenin was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241316 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241316 at 1 µg/ml.
Lane 1: ab241316 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Lysate prepared using NETN lysis buffer.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab241316 has not yet been referenced specifically in any publications.
