Overview

  • Product name

  • Description

    Rabbit polyclonal to Calumenin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Xenopus laevis, Zebrafish, Syrian hamster, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human Calumenin aa 1-50. The exact sequence is proprietary.
    Database link: O43852

  • Positive control

    • IP: HEK-293T whole cell lysate.
  • General notes

    ab241317 has not performed satisfactorily when used for WB of Calumenin in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Calumenin.

Properties

Applications

Our Abpromise guarantee covers the use of ab241317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    Images

    • Calumenin was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241317 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241317 at 1 µg/ml.

      Lane 1: ab241317 IP in HEK-293T whole cell lysate.

      Lane 2: Control IgG IP in HEK-293T whole cell lysate.

      Detection: Chemiluminescence with exposure time of 30 seconds.

      Lysate prepared using NETN lysis buffer.

    References

    ab241317 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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