Overview

  • Product name

    Anti-Calumenin antibody [EPR9075]
    See all Calumenin primary antibodies
  • Description

    Rabbit monoclonal [EPR9075] to Calumenin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Calumenin aa 250-350. The exact sequence is proprietary.

  • Positive control

    • Human placenta, Jurkat, 293T, K562, and HACAT lysates; Human placenta and urinary bladder transitional carcinoma tissues
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Involved in regulation of vitamin K-dependent carboxylation of multiple amino-terminal glutamate residues. Seems to inhibit gamma-carboxylase GGCX. Binds 7 calcium ions with a low affinity.
    • Tissue specificity

      Ubiquitously expressed. Expressed at high levels in heart, placenta and skeletal muscle, at lower levels in lung, kidney and pancreas and at very low levels in brain and liver.
    • Sequence similarities

      Belongs to the CREC family.
      Contains 6 EF-hand domains.
    • Cellular localization

      Endoplasmic reticulum lumen. Secreted. Melanosome. Sarcoplasmic reticulum lumen. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links

    • Alternative names

      • CALU antibody
      • CALU_HUMAN antibody
      • Calumenin 1 antibody
      • Calumenin 2 antibody
      • Calumenin antibody
      • Crocalbin antibody
      • crocalbin-like protein antibody
      • FLJ90608 antibody
      • IEF SSP 9302 antibody
      • multiple EF-hand protein antibody
      • OTTHUMP00000212712 antibody
      • OTTHUMP00000212713 antibody
      • OTTHUMP00000212714 antibody
      • OTTHUMP00000212715 antibody
      see all

    Images

    • All lanes : Anti-Calumenin antibody [EPR9075] (ab137019) at 1/1000 dilution

      Lane 1 : Human placenta lysate
      Lane 2 : Jurkat lysate
      Lane 3 : 293T lysate
      Lane 4 : K562 lysate
      Lane 5 : HACAT lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 37 kDa

    • Overlay histogram showing JEG3 cells stained with ab137019 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137019, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Immunohistochemical analysis of paraffin-embedded Human placanta tissue labelling Calumenin with ab137019 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human urinary bladder transitional carcinoma tissue labelling Calumenin with ab137019 at 1/100 dilution.

    References

    This product has been referenced in:

    • Kunita A  et al. MicroRNA-21 in cancer-associated fibroblasts supports lung adenocarcinoma progression. Sci Rep 8:8838 (2018). IHC-P ; Human . Read more (PubMed: 29892003) »
    • Wang Y  et al. PERK/CHOP contributes to the CGK733-induced vesicular calcium sequestration which is accompanied by non-apoptotic cell death. Oncotarget 6:25252-65 (2015). Read more (PubMed: 26259235) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Question
    Answer

    I can confirm that this antibody is produced by cells grown in serum-free media.

    Read More

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