Product nameAnti-Calumenin antibody [EPR9075]
See all Calumenin primary antibodies
DescriptionRabbit monoclonal [EPR9075] to Calumenin
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Calumenin aa 250-350. The exact sequence is proprietary.
- Human placenta, Jurkat, 293T, K562, and HACAT lysates; Human placenta and urinary bladder transitional carcinoma tissues
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab137019 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 37 kDa.|
|IHC-P||1/100 - 1/250.|
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionInvolved in regulation of vitamin K-dependent carboxylation of multiple amino-terminal glutamate residues. Seems to inhibit gamma-carboxylase GGCX. Binds 7 calcium ions with a low affinity.
Tissue specificityUbiquitously expressed. Expressed at high levels in heart, placenta and skeletal muscle, at lower levels in lung, kidney and pancreas and at very low levels in brain and liver.
Sequence similaritiesBelongs to the CREC family.
Contains 6 EF-hand domains.
Cellular localizationEndoplasmic reticulum lumen. Secreted. Melanosome. Sarcoplasmic reticulum lumen. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- CALU antibody
- CALU_HUMAN antibody
- Calumenin 1 antibody
All lanes : Anti-Calumenin antibody [EPR9075] (ab137019) at 1/1000 dilution
Lane 1 : Human placenta lysate
Lane 2 : Jurkat lysate
Lane 3 : 293T lysate
Lane 4 : K562 lysate
Lane 5 : HACAT lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 37 kDa
Overlay histogram showing JEG3 cells stained with ab137019 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137019, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunohistochemical analysis of paraffin-embedded Human placanta tissue labelling Calumenin with ab137019 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human urinary bladder transitional carcinoma tissue labelling Calumenin with ab137019 at 1/100 dilution.
This product has been referenced in:
- Kunita A et al. MicroRNA-21 in cancer-associated fibroblasts supports lung adenocarcinoma progression. Sci Rep 8:8838 (2018). IHC-P ; Human . Read more (PubMed: 29892003) »
- Wang Y et al. PERK/CHOP contributes to the CGK733-induced vesicular calcium sequestration which is accompanied by non-apoptotic cell death. Oncotarget 6:25252-65 (2015). Read more (PubMed: 26259235) »