Recombinant
RabMAb

Recombinant Anti-Calumenin antibody [EPR9075] - BSA and Azide free (ab240094)

Overview

  • Product name

    Anti-Calumenin antibody [EPR9075] - BSA and Azide free
    See all Calumenin primary antibodies
  • Description

    Rabbit monoclonal [EPR9075] to Calumenin - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Calumenin aa 250-350. The exact sequence is proprietary.

  • General notes

    Ab240094 is the carrier-free version of ab137019. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240094 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Involved in regulation of vitamin K-dependent carboxylation of multiple amino-terminal glutamate residues. Seems to inhibit gamma-carboxylase GGCX. Binds 7 calcium ions with a low affinity.
    • Tissue specificity

      Ubiquitously expressed. Expressed at high levels in heart, placenta and skeletal muscle, at lower levels in lung, kidney and pancreas and at very low levels in brain and liver.
    • Sequence similarities

      Belongs to the CREC family.
      Contains 6 EF-hand domains.
    • Cellular localization

      Endoplasmic reticulum lumen. Secreted. Melanosome. Sarcoplasmic reticulum lumen. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links

    • Alternative names

      • CALU antibody
      • CALU_HUMAN antibody
      • Calumenin 1 antibody
      • Calumenin 2 antibody
      • Calumenin antibody
      • Crocalbin antibody
      • crocalbin-like protein antibody
      • FLJ90608 antibody
      • IEF SSP 9302 antibody
      • multiple EF-hand protein antibody
      • OTTHUMP00000212712 antibody
      • OTTHUMP00000212713 antibody
      • OTTHUMP00000212714 antibody
      • OTTHUMP00000212715 antibody
      see all

    Images

    • Overlay histogram showing JEG3 cells stained with ab137019 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137019, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137019).

    • Immunohistochemical analysis of paraffin-embedded Human placanta tissue labelling Calumenin with ab137019 at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137019).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human urinary bladder transitional carcinoma tissue labelling Calumenin with ab137019 at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137019).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    ab240094 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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