Key features and details
- Rabbit polyclonal to CAMK1D
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CAMK1D antibody
See all CAMK1D primary antibodies
DescriptionRabbit polyclonal to CAMK1D
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Zebrafish
Recombinant fragment, corresponding to a sequence within amino acids 1-355 of Human CAMK1D (AAH35745).
- WB: 293T, HeLaS3, HepG2, MOLT4 or Raji whole cell lysate; ICC/IF: HeLa cells; IHC-P: OVCAR3 xenograft tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97803 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 43 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||1/100 - 1/200.|
FunctionCalcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May regulate calcium-mediated granulocyte function. May play a role in apoptosis of erythroleukemia cells. Activates MAP kinase MAPK3 (By similarity). In vitro, phosphorylates transcription factor CREM isoform Beta and probably CREB1.
Tissue specificityBroadly expressed. Highly and mostly expressed in polymorphonuclear leukocytes (neutrophilic and eosinophilic granulocytes) while little or no expression is observed in monocytes and lymphocytes.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.
DomainThe autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.
Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic (Probable). Also nuclear upon activation.
- Information by UniProt
- A630059D12Rik antibody
- Calcium/calmodulin dependent protein kinase ID antibody
- Calcium/calmodulin dependent protein kinase type 1D antibody
Anti-CAMK1D antibody (ab97803) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 43 kDa
Top: ab97803 at 1/200 dilution staining CAMK1D in PFA-fixed HeLa cells by immunofluorescence.
Bottom: image merged with DNA probe.
ab97803 at 1/500 dilution staining CAMK1D in paraffin-embedded OVCAR3 xenograft by immunohistochemistry.
ab97803 has not yet been referenced specifically in any publications.