Product nameAnti-CAMK1D antibody [EPR3536(2)]
See all CAMK1D primary antibodies
DescriptionRabbit monoclonal [EPR3536(2)] to CAMK1D
Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CAMK1D aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q8IU85
- Human fetal forebrain, 293T and HeLa lysates; 293T cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab172618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa).|
|ICC/IF||1/50 - 1/100.|
FunctionCalcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May regulate calcium-mediated granulocyte function. May play a role in apoptosis of erythroleukemia cells. Activates MAP kinase MAPK3 (By similarity). In vitro, phosphorylates transcription factor CREM isoform Beta and probably CREB1.
Tissue specificityBroadly expressed. Highly and mostly expressed in polymorphonuclear leukocytes (neutrophilic and eosinophilic granulocytes) while little or no expression is observed in monocytes and lymphocytes.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.
DomainThe autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.
Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic (Probable). Also nuclear upon activation.
- Information by UniProt
- A630059D12Rik antibody
- Calcium/calmodulin dependent protein kinase ID antibody
- Calcium/calmodulin dependent protein kinase type 1D antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: CAMK1D knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HEK293 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab172618 observed at 43 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab172618 was shown to specifically react with CAMK1D when CAMK1D knockout samples were used. Wild-type and CAMK1D knockout samples were subjected to SDS-PAGE. ab172618 and ab18058 (loading control to Vinculin) were both diluted 1/10,000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-CAMK1D antibody [EPR3536(2)] (ab172618) at 1/10000 dilution
Lane 1 : Human fetal forebrain tissue lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 43 kDa
Immunofluorescent analysis of 293T cells labeling CAMK1D with ab172618 at 1/50 dilution (red). DAPI nuclear staining (blue).