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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CAMK1D aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q8IU85
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab172618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa).|
|ICC/IF||1/50 - 1/100.|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: CAMK1D knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HEK293 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab172618 observed at 43 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab172618 was shown to specifically react with CAMK1D when CAMK1D knockout samples were used. Wild-type and CAMK1D knockout samples were subjected to SDS-PAGE. ab172618 and ab18058 (loading control to Vinculin) were both diluted 1/10,000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunofluorescent analysis of 293T cells labeling CAMK1D with ab172618 at 1/50 dilution (red). DAPI nuclear staining (blue).
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