Overview

  • Product name
    Anti-CAMK1D antibody [EPR3536(2)]
    See all CAMK1D primary antibodies
  • Description
    Rabbit monoclonal [EPR3536(2)] to CAMK1D
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CAMK1D aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q8IU85

  • Positive control
    • Human fetal forebrain, 293T and HeLa lysates; 293T cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab172618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa).
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May regulate calcium-mediated granulocyte function. May play a role in apoptosis of erythroleukemia cells. Activates MAP kinase MAPK3 (By similarity). In vitro, phosphorylates transcription factor CREM isoform Beta and probably CREB1.
    • Tissue specificity
      Broadly expressed. Highly and mostly expressed in polymorphonuclear leukocytes (neutrophilic and eosinophilic granulocytes) while little or no expression is observed in monocytes and lymphocytes.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
      Contains 1 protein kinase domain.
    • Domain
      The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.
    • Cellular localization
      Cytoplasm. Nucleus. Predominantly cytoplasmic (Probable). Also nuclear upon activation.
    • Information by UniProt
    • Database links
    • Alternative names
      • A630059D12Rik antibody
      • Calcium/calmodulin dependent protein kinase ID antibody
      • Calcium/calmodulin dependent protein kinase type 1D antibody
      • Calcium/calmodulin-dependent protein kinase I-delta antibody
      • Calcium/calmodulin-dependent protein kinase type 1D antibody
      • CaM K1 antibody
      • CaM KI delta antibody
      • CaM kinase I delta antibody
      • CaM kinase ID antibody
      • CaM-KI delta antibody
      • CAMK 1D antibody
      • CAMK1D antibody
      • CaMKI delta antibody
      • CamKI like protein kinase antibody
      • CamKI-like protein kinase antibody
      • CaMKID antibody
      • CKLiK antibody
      • E030025C11Rik antibody
      • KCC1D_HUMAN antibody
      • OTTHUMP00000019119 antibody
      • OTTHUMP00000019120 antibody
      • OTTHUMP00000045030 antibody
      • RP11 462F15.1 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: CAMK1D knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: HEK293 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab172618 observed at 43 kDa. Red - loading control, ab18058, observed at 124 kDa.

      ab172618 was shown to specifically react with CAMK1D when CAMK1D knockout samples were used. Wild-type and CAMK1D knockout samples were subjected to SDS-PAGE. ab172618 and ab18058 (loading control to Vinculin) were both diluted 1/10,000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-CAMK1D antibody [EPR3536(2)] (ab172618) at 1/10000 dilution

      Lane 1 : Human fetal forebrain tissue lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 43 kDa

    • Immunofluorescent analysis of 293T cells labeling CAMK1D with ab172618 at 1/50 dilution (red). DAPI nuclear staining (blue).

       

    References

    This product has been referenced in:
    • Alfaradhi MZ  et al. Maternal Obesity in Pregnancy Developmentally Programs Adipose Tissue Inflammation in Young, Lean Male Mice Offspring. Endocrinology 157:4246-4256 (2016). WB ; Mouse . Read more (PubMed: 27583789) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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