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Overview
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Product nameAnti-CaMK2 beta gamma delta antibody
See all CaMK2 beta gamma delta primary antibodies -
DescriptionRabbit polyclonal to CaMK2 beta gamma delta
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Host speciesRabbit
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SpecificityAntibody detects endogenous levels of total CaMK2-beta/gamma/delta protein.
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Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthesized non-phosphopeptide derived from human CaMK2-beta/gamma/delta around the phosphorylation site of threonine 287 (Q-E-TP-V-E).
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Positive control
- Human brain tissue and rat brain cell extract
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab79359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/5000. | |
| WB | 1/500 - 1/1000. Predicted molecular weight: 73 kDa. | |
| IHC-P | 1/50 - 1/100. |
Target
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RelevanceThe product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Eight transcript variants encoding eight distinct isoforms have been identified for this gene.
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Cellular localizationCytoplasmic and Nuclear
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Database links
- Entrez Gene: 816 Human
- Entrez Gene: 817 Human
- Entrez Gene: 818 Human
- Entrez Gene: 108058 Mouse
- Entrez Gene: 12323 Mouse
- Entrez Gene: 12325 Mouse
- Entrez Gene: 171140 Rat
- Entrez Gene: 24245 Rat
see all -
Alternative names
- Calcium/calmodulin-dependent protein antibody
- CaM kinase II beta subunit antibody
- CaM-kinase II beta chain antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMK2 beta gamma delta antibody (ab79359)Immunohistochemistry analysis of paraffin-embedded human brain tissue using CaMK2-ß/?/d (Ab-287) antibody at 1/50-1/100 dilution. -
Western blot - Anti-CaMK2 beta gamma delta antibody (ab79359)All lanes : Anti-CaMK2 beta gamma delta antibody (ab79359) at 1/500 dilution
Lane 1 : Rat brain cell extract
Lane 2 : Rat brain cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 73 kDa
Observed band size: ~73 kDa (why is the actual band size different from the predicted?)
Additional bands at: ~50 kDa. We are unsure as to the identity of these extra bands.
References
ab79359 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"