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Neuroscience Neurotransmission Calcium Signaling Calmodulin / CaMK
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Anti-CaMK2 beta gamma delta antibody (ab79359)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMK2 beta gamma delta antibody (ab79359)
  • Western blot - Anti-CaMK2 beta gamma delta antibody (ab79359)

Key features and details

  • Rabbit polyclonal to CaMK2 beta gamma delta
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-CaMK2 beta gamma delta antibody
    See all CaMK2 beta gamma delta primary antibodies
  • Description

    Rabbit polyclonal to CaMK2 beta gamma delta
  • Host species

    Rabbit
  • Specificity

    Antibody detects endogenous levels of total CaMK2-beta/gamma/delta protein.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthesized non-phosphopeptide derived from human CaMK2-beta/gamma/delta around the phosphorylation site of threonine 287 (Q-E-TP-V-E).

  • Positive control

    • Human brain tissue and rat brain cell extract
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • Calmodulin / CaMK
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calmodulin Pathway
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab79359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/1000. Predicted molecular weight: 73 kDa.
IHC-P
1/50 - 1/100.
Notes
WB
1/500 - 1/1000. Predicted molecular weight: 73 kDa.
IHC-P
1/50 - 1/100.

Target

  • Relevance

    The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Eight transcript variants encoding eight distinct isoforms have been identified for this gene.
  • Cellular localization

    Cytoplasmic and Nuclear
  • Database links

    • Entrez Gene: 816 Human
    • Entrez Gene: 817 Human
    • Entrez Gene: 818 Human
    • Entrez Gene: 108058 Mouse
    • Entrez Gene: 12323 Mouse
    • Entrez Gene: 12325 Mouse
    • Entrez Gene: 171140 Rat
    • Entrez Gene: 24245 Rat
    • Entrez Gene: 24246 Rat
    • Omim: 114078 Human
    • Omim: 607707 Human
    • Omim: 608721 Human
    • SwissProt: Q13554 Human
    • SwissProt: Q13555 Human
    • SwissProt: Q13557 Human
    • SwissProt: P28652 Mouse
    • SwissProt: Q6PHZ2 Mouse
    • SwissProt: Q923T9 Mouse
    • SwissProt: P08413 Rat
    • SwissProt: P11730 Rat
    • SwissProt: P15791 Rat
    see all
  • Alternative names

    • Calcium/calmodulin-dependent protein antibody
    • CaM kinase II beta subunit antibody
    • CaM-kinase II beta chain antibody
    • CAM2 antibody
    • CAMK antibody
    • CAMK-II antibody
    • CaMK-II beta subunit antibody
    • CAMK2B antibody
    • CAMK2D antibody
    • CAMK2G antibody
    • CAMK2N2 antibody
    • CAMKB antibody
    • CAMKD antibody
    • CAMKG antibody
    • KCC2B antibody
    • kinase CaMK2-beta antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMK2 beta gamma delta antibody (ab79359)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMK2 beta gamma delta antibody (ab79359)
    Immunohistochemistry analysis of paraffin-embedded human brain tissue using CaMK2-ß/?/d (Ab-287) antibody at 1/50-1/100 dilution.
  • Western blot - Anti-CaMK2 beta gamma delta antibody (ab79359)
    Western blot - Anti-CaMK2 beta gamma delta antibody (ab79359)
    All lanes : Anti-CaMK2 beta gamma delta antibody (ab79359) at 1/500 dilution

    Lane 1 : Rat brain cell extract
    Lane 2 : Rat brain cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 73 kDa
    Observed band size: ~73 kDa why is the actual band size different from the predicted?
    Additional bands at: ~50 kDa. We are unsure as to the identity of these extra bands.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab79359? Please let us know so that we can cite the reference in this datasheet.

ab79359 has not yet been referenced specifically in any publications.

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