Anti-CaMKI antibody (ab137409)
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- References
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Overview
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Product name
Anti-CaMKI antibody
See all CaMKI primary antibodies -
Description
Rabbit polyclonal to CaMKI -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Xenopus laevis, Xenopus tropicalis
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 99-312 of Human CaMKI.
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Positive control
- HepG2 whole cell lysate. HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab137409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 41 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca(2+) elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on 'Ser-694', which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on 'Ser-91', 'Ser-92', 'Ser-93' and 'Ser-294'. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of 'Ser-259' and 'Ser-498' in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on 'Ser-276' and 'Ser-295'. Involved in the regulation of basal and estrogen-stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation (By similarity). Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K(+) and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I. -
Tissue specificity
Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. -
Domain
The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate. -
Post-translational
modificationsPhosphorylated by CaMKK1 and CaMKK2 on Thr-177.
Polybiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL12), leading to proteasomal degradation. -
Cellular localization
Cytoplasm. Nucleus. Predominantly cytoplasmic. - Information by UniProt
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Database links
- Entrez Gene: 520498 Cow
- Entrez Gene: 8536 Human
- Entrez Gene: 52163 Mouse
- Entrez Gene: 100156272 Pig
- Entrez Gene: 171503 Rat
- Entrez Gene: 379971 Xenopus laevis
- Entrez Gene: 398402 Xenopus laevis
- Entrez Gene: 549023 Xenopus tropicalis
see all -
Alternative names
- Calcium / calmodulin dependent protein kinase 1 antibody
- Calcium / calmodulin dependent protein kinase I antibody
- Calcium / calmodulin dependent protein kinase type 1 antibody
see all
Images
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Western blot - Anti-CaMKI antibody (ab137409)Anti-CaMKI antibody (ab137409) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 41 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-CaMKI antibody (ab137409)Immunofluorescent analysis of methanol-fixed HeLa cells labelling CaMKI with ab137409 at 1/200 dilution. Lower panel is costained with Hoechst 33342.
References
ab137409 has not yet been referenced specifically in any publications.
