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Neuroscience Neurotransmission Calcium Signaling Calmodulin / CaMK
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Anti-CaMKI antibody (ab3551)

  • Datasheet
  • SDS
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Western blot - Anti-CaMKI antibody (ab3551)

    Key features and details

    • Rabbit polyclonal to CaMKI
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    You may also be interested in

    Secondary
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    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
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    Recombinant mouse CaMKI protein (ab51398)

    View more associated products

    Overview

    • Product name

      Anti-CaMKI antibody
      See all CaMKI primary antibodies
    • Description

      Rabbit polyclonal to CaMKI
    • Host species

      Rabbit
    • Specificity

      Detects CaMKI from rat brain extract.
    • Tested Applications & Species

      Application Species
      WB
      Mouse
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Human CaMKI aa 1-100 (N terminal).
      (Peptide available as ab5015)

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • WB: AtT20 cell extract, rat brain extract.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.05% Sodium azide
      Constituents: 0.1% BSA, 99% PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurotransmission
      • Calcium Signaling
      • Calmodulin / CaMK
      • Signal Transduction
      • Signaling Pathway
      • Calcium Signaling
      • Calmodulin Pathway
      • Cardiovascular
      • Heart
      • Hypertrophy
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Immunizing Peptide (Blocking)

      • CaMKI peptide (ab5015)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant mouse CaMKI protein (ab51398)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab3551 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Mouse
    All applications
    Human
    Application Abreviews Notes
    WB
    Use a concentration of 2 µg/ml. Predicted molecular weight: 41 kDa.Can be blocked with CaMKI peptide (ab5015).

    This antibody detects an ~43 kDa protein representing CaMKI from rat brain extract.

    Notes
    WB
    Use a concentration of 2 µg/ml. Predicted molecular weight: 41 kDa.Can be blocked with CaMKI peptide (ab5015).

    This antibody detects an ~43 kDa protein representing CaMKI from rat brain extract.

    Target

    • Function

      Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca(2+) elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on 'Ser-694', which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on 'Ser-91', 'Ser-92', 'Ser-93' and 'Ser-294'. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of 'Ser-259' and 'Ser-498' in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on 'Ser-276' and 'Ser-295'. Involved in the regulation of basal and estrogen-stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation (By similarity). Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K(+) and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I.
    • Tissue specificity

      Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex.
    • Sequence similarities

      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
      Contains 1 protein kinase domain.
    • Domain

      The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.
    • Post-translational
      modifications

      Phosphorylated by CaMKK1 and CaMKK2 on Thr-177.
      Polybiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL12), leading to proteasomal degradation.
    • Cellular localization

      Cytoplasm. Nucleus. Predominantly cytoplasmic.
    • Target information above from: UniProt accession Q14012 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 8536 Human
      • Entrez Gene: 52163 Mouse
      • Omim: 604998 Human
      • SwissProt: Q14012 Human
      • SwissProt: Q91YS8 Mouse
      • Unigene: 434875 Human
      • Unigene: 277373 Mouse
      • Alternative names

        • Calcium / calmodulin dependent protein kinase 1 antibody
        • Calcium / calmodulin dependent protein kinase I antibody
        • Calcium / calmodulin dependent protein kinase type 1 antibody
        • Calcium/calmodulin dependent protein kinase 1 antibody
        • Calcium/calmodulin dependent protein kinase I antibody
        • Calcium/calmodulin dependent protein kinase type 1 antibody
        • Calcium/calmodulin-dependent protein kinase type 1 antibody
        • CaM K1 antibody
        • CaM KI antibody
        • CaM kinase 1 alpha antibody
        • CAM kinase 1 antibody
        • CaM kinase I alpha antibody
        • CaM kinase I antibody
        • CaM-KI antibody
        • CaMK 1 antibody
        • CAMK I antibody
        • CaMK1 alpha antibody
        • CAMK1 antibody
        • CAMK1 PEN antibody
        • CaMKI alpha antibody
        • CaMKI-alpha antibody
        • KCC1A_HUMAN antibody
        • MGC120317 antibody
        • MGC120318 antibody
        see all

      Images

      • Western blot - Anti-CaMKI antibody (ab3551)
        Western blot - Anti-CaMKI antibody (ab3551)
        Anti-CaMKI antibody (ab3551) at 2 µg/ml + Cell lysate prepared from AtT20 cells

        Predicted band size: 41 kDa



        ab3551 at a 2µg/ml concentration staining CaMKI in AtT20 cell lysate by Western blot.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab3551? Please let us know so that we can cite the reference in this datasheet.

      ab3551 has been referenced in 1 publication.

      • Zhang X  et al. Calcium/calmodulin-dependent protein kinase (CaMK) Ialpha mediates the macrophage inflammatory response to sepsis. J Leukoc Biol 90:249-61 (2011). WB ; Mouse . PubMed: 21372190

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