Anti-CaMKI antibody [EP2218Y] (ab62374)
![Western blot - Anti-CaMKI antibody [EP2218Y] (ab62374)](https://www.abcam.com/ps/products/62/ab62374/Images/ab62374-75703-anti-camki-antibody-ep2218y-western-blot.gif "Western blot - Anti-CaMKI antibody [EP2218Y] (ab62374)")
Key features and details
- Rabbit monoclonal [EP2218Y] to CaMKI
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CaMKI antibody [EP2218Y]
See all CaMKI primary antibodies -
Description
Rabbit monoclonal [EP2218Y] to CaMKI -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human CaMKI (C terminal). The exact sequence is proprietary.
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Positive control
- SH-SY5Y cell lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2218Y -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62374 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
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| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/20000 - 1/50000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
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| Notes |
|---|
|
WB
1/20000 - 1/50000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
Target
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Function
Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca(2+) elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on 'Ser-694', which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on 'Ser-91', 'Ser-92', 'Ser-93' and 'Ser-294'. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of 'Ser-259' and 'Ser-498' in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on 'Ser-276' and 'Ser-295'. Involved in the regulation of basal and estrogen-stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation (By similarity). Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K(+) and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I. -
Tissue specificity
Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. -
Domain
The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate. -
Post-translational
modificationsPhosphorylated by CaMKK1 and CaMKK2 on Thr-177.
Polybiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL12), leading to proteasomal degradation. -
Cellular localization
Cytoplasm. Nucleus. Predominantly cytoplasmic. - Information by UniProt
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Database links
- Entrez Gene: 8536 Human
- Omim: 604998 Human
- SwissProt: Q14012 Human
- Unigene: 434875 Human
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Alternative names
- Calcium / calmodulin dependent protein kinase 1 antibody
- Calcium / calmodulin dependent protein kinase I antibody
- Calcium / calmodulin dependent protein kinase type 1 antibody
see all
Images
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Western blot - Anti-CaMKI antibody [EP2218Y] (ab62374)Anti-CaMKI antibody [EP2218Y] (ab62374) at 1/20000 dilution + SH-SY5Y cell lysate at 10 µg
Secondary
goat anti-rabbit, HRP labeled, at 1/2000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab62374 has been referenced in 1 publication.
- Pfisterer SG et al. Ca2+/calmodulin-dependent kinase (CaMK) signaling via CaMKI and AMP-activated protein kinase contributes to the regulation of WIPI-1 at the onset of autophagy. Mol Pharmacol 80:1066-75 (2011). WB ; Human . PubMed: 21896713