CaMKI peptide (ab5015)

Submit a review Submit a question

Key features and details

  • Suitable for: Blocking

Description

Specifications

Our Abpromise guarantee covers the use of ab5015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking

  • Form

    Lyophilized

  • Additional notes


    This peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and human CaMKI protein, catalog ab3551. Using a solution of peptide of equal volume and concentration to the corresponding antibody will yield a large molar excess of peptide (~ 70-fold) for competitive inhibition of antibody-protein binding reactions.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Reconstitution
    >95% pure, lyophilized synthetic peptide. Reconstitute with 0.1 ml of distilled water.

General Info

  • Alternative names

    • Calcium / calmodulin dependent protein kinase 1
    • Calcium / calmodulin dependent protein kinase I
    • Calcium / calmodulin dependent protein kinase type 1
    • Calcium/calmodulin dependent protein kinase 1
    • Calcium/calmodulin dependent protein kinase I
    • Calcium/calmodulin dependent protein kinase type 1
    • Calcium/calmodulin-dependent protein kinase type 1
    • CaM K1
    • CaM KI
    • CAM kinase 1
    • CaM kinase 1 alpha
    • CaM kinase I
    • CaM kinase I alpha
    • CaM-KI
    • CaMK 1
    • CAMK I
    • CAMK1
    • CaMK1 alpha
    • CAMK1 PEN
    • CaMKI alpha
    • CaMKI-alpha
    • KCC1A_HUMAN
    • MGC120317
    • MGC120318
    see all

  • Function

    Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca(2+) elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on 'Ser-694', which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on 'Ser-91', 'Ser-92', 'Ser-93' and 'Ser-294'. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of 'Ser-259' and 'Ser-498' in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on 'Ser-276' and 'Ser-295'. Involved in the regulation of basal and estrogen-stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation (By similarity). Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K(+) and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I.

  • Tissue specificity

    Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex.

  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.

  • Domain

    The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.

  • Post-translational
    modifications

    Phosphorylated by CaMKK1 and CaMKK2 on Thr-177.
    Polybiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL12), leading to proteasomal degradation.

  • Cellular localization

    Cytoplasm. Nucleus. Predominantly cytoplasmic.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab5015? Please let us know so that we can cite the reference in this datasheet.

ab5015 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab5015.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com