Anti-CaMKI (phospho T177) antibody (ab62215)
Key features and details
- Rabbit polyclonal to CaMKI (phospho T177)
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CaMKI (phospho T177) antibody
See all CaMKI primary antibodies -
Description
Rabbit polyclonal to CaMKI (phospho T177) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human CaMKI aa 100-200 (phospho T177).
Database link: Q14012 -
Positive control
- HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab62215 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 4 µg/ml.
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ICC/IF |
1/500 - 1/1000.
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Notes |
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IHC-P
Use a concentration of 4 µg/ml. |
ICC/IF
1/500 - 1/1000. |
Target
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Function
Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca(2+) elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on 'Ser-694', which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on 'Ser-91', 'Ser-92', 'Ser-93' and 'Ser-294'. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of 'Ser-259' and 'Ser-498' in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on 'Ser-276' and 'Ser-295'. Involved in the regulation of basal and estrogen-stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation (By similarity). Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K(+) and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I. -
Tissue specificity
Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. -
Domain
The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate. -
Post-translational
modificationsPhosphorylated by CaMKK1 and CaMKK2 on Thr-177.
Polybiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL12), leading to proteasomal degradation. -
Cellular localization
Cytoplasm. Nucleus. Predominantly cytoplasmic. - Information by UniProt
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Database links
- Entrez Gene: 8536 Human
- Entrez Gene: 52163 Mouse
- Entrez Gene: 171503 Rat
- Omim: 604998 Human
- SwissProt: Q14012 Human
- SwissProt: Q91YS8 Mouse
- SwissProt: Q63450 Rat
- Unigene: 434875 Human
see all -
Alternative names
- Calcium / calmodulin dependent protein kinase 1 antibody
- Calcium / calmodulin dependent protein kinase I antibody
- Calcium / calmodulin dependent protein kinase type 1 antibody
see all
Images
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Immunofluorescence analysis of HeLa cells, using ab62215 (1/500 - 1/1000) staining CaMKI; in the presence and absence of immunizing peptide.
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Ab62215 staining human normal occipital cortex. Staining is localised to nuclear and cytoplasmic compartments.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and ampli
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab62215 has been referenced in 6 publications.
- Yang J et al. Pitavastatin activates mitophagy to protect EPC proliferation through a calcium-dependent CAMK1-PINK1 pathway in atherosclerotic mice. Commun Biol 5:124 (2022). PubMed: 35145192
- Suzuki Y et al. Local Ca2+ Signals within Caveolae Cause Nuclear Translocation of CaMK1α in Mouse Vascular Smooth Muscle Cells. Biol Pharm Bull 45:1354-1363 (2022). PubMed: 36047205
- Li M et al. Transient Receptor Potential V Channels Are Essential for Glucose Sensing by Aldolase and AMPK. Cell Metab 30:508-524.e12 (2019). PubMed: 31204282
- Takano T et al. Discovery of long-range inhibitory signaling to ensure single axon formation. Nat Commun 8:33 (2017). PubMed: 28652571
- Kotla S et al. Heterodimers of the transcriptional factors NFATc3 and FosB mediate tissue factor expression for 15(S)-hydroxyeicosatetraenoic acid-induced monocyte trafficking. J Biol Chem 292:14885-14901 (2017). PubMed: 28724635
- Tinsley CJ et al. A role for the CAMKK pathway in visual object recognition memory. Hippocampus 22:466-76 (2012). PubMed: 21298728