Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1830] to CaMKII alpha + CaMKII beta (phospho T286)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-CaMKII alpha + CaMKII beta (phospho T286) antibody [EPR1830]
See all CaMKII alpha + CaMKII beta (phospho T286) primary antibodies
DescriptionRabbit monoclonal [EPR1830] to CaMKII alpha + CaMKII beta (phospho T286)
SpecificityDue to the homology between the immunogen peptide and other CAMKII isoforms, ab124880 may cross react with CAMKII delta (KCC2D, 93% homology) and CAMKII gamma (KCC2G, 79% homology)
Tested applicationsSuitable for: WBmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CaMKII alpha + CaMKII beta (phospho T286) aa 250-350.
- Rat brain lysate (cells untreated/treated with Lambda Phosphatase).
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 3.60 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124880 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 45-70 kDa (predicted molecular weight: 54 kDa).|
RelevanceFunction: CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. Tissue specificity: Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain.
- Alpha CaMKII antibody
- Calcium calmodulin dependent protein kinase II antibody
- Calcium/calmodulin dependent protein kinase (CaM kinase) II beta antibody
All lanes : Anti-CaMKII alpha + CaMKII beta (phospho T286) antibody [EPR1830] (ab124880) at 1/1000 dilution
Lane 1 : Rat brain lysate untreated
Lane 2 : Rat brain lysate treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 54 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab124880 has been referenced in 8 publications.
- Xie W et al. Antidepressant-like effects of the Guanxin Danshen formula via mediation of the CaMK II-CREB-BDNF signalling pathway in chronic unpredictable mild stress-induced depressive rats. Ann Transl Med 7:564 (2019). PubMed: 31807545
- Zhu A et al. Wnt5a mediates chronic post-thoracotomy pain by regulating non-canonical pathways, nerve regeneration, and inflammation in rats. Cell Signal 44:51-61 (2018). WB . PubMed: 29339085
- Zhang L et al. Curcumin induces endoplasmic reticulum stress-associated apoptosis in human papillary thyroid carcinoma BCPAP cells via disruption of intracellular calcium homeostasis. Medicine (Baltimore) 97:e11095 (2018). PubMed: 29901626
- Zhang F et al. cTAGE5/MEA6 plays a critical role in neuronal cellular components trafficking and brain development. Proc Natl Acad Sci U S A 115:E9449-E9458 (2018). PubMed: 30224460
- Ahmed ME et al. Beneficial Effects of a CaMKIIa Inhibitor TatCN21 Peptide in Global Cerebral Ischemia. J Mol Neurosci 61:42-51 (2017). PubMed: 27604243
- Yanpallewar S et al. Nedd4-2 haploinsufficiency causes hyperactivity and increased sensitivity to inflammatory stimuli. Sci Rep 6:32957 (2016). WB . PubMed: 27604420
- La Montanara P et al. Synaptic synthesis, dephosphorylation, and degradation: a novel paradigm for an activity-dependent neuronal control of CDKL5. J Biol Chem 290:4512-27 (2015). PubMed: 25555910
- Haolong C et al. Enterovirus 71 VP1 activates calmodulin-dependent protein kinase II and results in the rearrangement of vimentin in human astrocyte cells. PLoS One 8:e73900 (2013). WB ; Human . PubMed: 24073199