Key features and details
- Rabbit polyclonal to CaMKII alpha
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-CaMKII alpha antibody
See all CaMKII alpha primary antibodies
DescriptionRabbit polyclonal to CaMKII alpha
SpecificityThis antibody does cross-react with CAMKII beta protein
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
- Human hippocampal region tissue; extracts from mouse brain tissue.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab131468 was purified by affinity-chromatography using epitope-specific peptide.
Our Abpromise guarantee covers the use of ab131468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).|
|ICC/IF||1/100 - 1/200.|
FunctionCaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.
Cellular localizationCell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts.
- Information by UniProt
- Alpha CaMKII antibody
- Calcium calmodulin dependent protein kinase II antibody
- Calcium/calmodulin dependent protein kinase II alpha B subunit antibody
All lanes : Anti-CaMKII alpha antibody (ab131468) at 1/500 dilution
Lane 1 : Extracts from mouse brain tissue
Lane 2 : Extracts from mouse brain tissue with blocking peptide
Predicted band size: 54 kDa
Immunofluorescent analysis of paraffin-embedded Human hippocampal region tissue from the falling sickness disease labelling CaMKII alpha with ab131468 at 1/100 dilution.
ab131468 has been referenced in 3 publications.
- Alexander GM et al. CA2 neuronal activity controls hippocampal low gamma and ripple oscillations. Elife 7:N/A (2018). PubMed: 30387713
- Lu Y et al. Intracellular Ca2+ homeostasis and JAK1/STAT3 pathway are involved in the protective effect of propofol on BV2 microglia against hypoxia-induced inflammation and apoptosis. PLoS One 12:e0178098 (2017). WB . PubMed: 28542400
- Shinsuke K et al. Chronic irradiation with low-dose-rate 137Cs-? rays inhibits NGF-induced neurite extension of PC12 cells via Ca2+/calmodulin-dependent kinase II activation. J Radiat Res 58:809-815 (2017). PubMed: 29106600