Western blot abreview for Anti-CaMKII alpha antibody

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Loading amount
5 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Dilution
1/8000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% milk

Secondary antibody

Name
Non-Abcam antibody was used: Amersham ECL Rabbit IgG, HRP-linked whole Ab
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 50 kDa
Positive control
Standard

Additional data

Additional Notes
Operator: Larysa Teplytska

Ms. Christiane Rewerts

Verified customer

Submitted Mar 31 2009

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