Overview

  • Product name

    Anti-CaMKII alpha antibody
    See all CaMKII alpha primary antibodies
  • Description

    Goat polyclonal to CaMKII alpha
  • Host species

    Goat
  • Specificity

    Expected to recognize both reported isoforms of CaMKII (NP_057065.2 and NP_741960.1). This antibody does cross-react with CAMKII beta protein
  • Tested applications

    Suitable for: IHC-P, WB, IHC-FoFrmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Dog
  • Immunogen

    Peptide with sequence

    C-PRTAQSEETRVWHR

    , corresponding to internal sequence amino acids 445-458 of human CaMKII alpha protein (NP_057065.2; NP_741960.1). (Peptide available as ab126462.)

  • Positive control

    • Human brain (cerebellum) and mouse brain lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab87597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 - 6 µg/ml.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 55, 65 kDa (predicted molecular weight: 55 kDa).Can be blocked with Human CaMKII alpha peptide (ab126462).
IHC-FoFr Use at an assay dependent concentration. PubMed: 24231734

Target

  • Function

    CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization

    Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha CaMKII antibody
    • Calcium calmodulin dependent protein kinase II antibody
    • Calcium/calmodulin dependent protein kinase II alpha B subunit antibody
    • Calcium/calmodulin dependent protein kinase type II alpha chain antibody
    • Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha antibody
    • Calcium/calmodulin-dependent protein kinase II alpha antibody
    • Calcium/calmodulin-dependent protein kinase II-alpha antibody
    • Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody
    • Calcium/calmodulin-dependent protein kinase type IIA antibody
    • CaM kinase II alpha chain antibody
    • CaM kinase II alpha subunit antibody
    • CaM kinase II subunit alpha antibody
    • CaMK II alpha subunit antibody
    • CaMK-II subunit alpha antibody
    • Camk2a antibody
    • CAMKA antibody
    • CaMKII antibody
    • CaMKIINalpha antibody
    • EC 2.7.11.17 antibody
    • KCC2A_HUMAN antibody
    • KIAA0968 antibody
    • MGC123320 antibody
    • MGC139375 antibody
    • MGC155201 antibody
    • mKIAA0968 antibody
    • PK2CDD antibody
    • PKCCD antibody
    • R74975 antibody
    • zgc:112538 antibody
    • zgc:123320 antibody
    see all

Images

  • Anti-CaMKII alpha antibody (ab87597) at 0.1 µg/ml + mouse brain lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa
    Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.

  • ab87597 at 3.8 µg/ml staining CaMKII in Human brain cortex. Steamed antigen retrieval with citrate buffer pH 6 was performed. Antibody was revealed with AP-staining.

References

This product has been referenced in:

  • Fraize N  et al. Differential changes in hippocampal CaMKII and GluA1 activity after memory training involving different levels of adaptive forgetting. Learn Mem 24:86-94 (2017). WB ; Rat . Read more (PubMed: 28096498) »
  • Gan JO  et al. Adolescent social isolation enhances the plasmalemmal density of NMDA NR1 subunits in dendritic spines of principal neurons in the basolateral amygdala of adult mice. Neuroscience 258:174-83 (2014). IHC-FoFr ; Mouse . Read more (PubMed: 24231734) »
See all 3 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Normal donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Antigen retrieval step
None
Sample
Rat Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 30 2014

Application
Immunohistochemistry (Frozen sections)
Sample
Cat Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Aug 13 2012

Answer

I am very pleased to hear you would like to accept our offer and test ab87597 in IHC-Fr. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IHC-Fr and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you very much for your interest in ab87597.

To our knowledge, ab87597 has not been tested in IHC-Fr. Therefore, I can offer a discount off a future purchase if you buy ab87597 now, test it in IHC-Fr and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free PRIMARY ANTIBODY OR VALUE OFF OF NEXT ORDER.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab87597 in IHC-Fr. I will then send a discount code. This code must be issued before purchasing ab87597 so please wait for my reply before ordering.

2. Purchase ab87597 either by phone, fax, or online (www.abcam.com).

3. Test it in IHC-Fr.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab87597 turns out to be unsuitable for IHC-Fr, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Answer

Vielen Dank für Ihre Anfrage.

Ich kann empfehlen den ab87597 mit einer Verdünnungreihe, ausgehend von 1/100 )zum Beispiel 1/50, 1/100 und 1/200) auszutested. Auch kann ich empfehlen, die Antigendemaskierung mit einem Citrate Puffer pH 6 unter Hitze vorzunehmen. In der Tat gab dieser Antikörper gute Resultate wenn er benutzt wurder mit 3.8 µg/ml auf human Hirncortex Gewebe. Wir empfehlen jedoch immer, den Antikörper in Ihren Konditionen und auf dem von Ihnen benutzten Gewebe auch zu optimisieren.
Der ab105502 wurder erfolgreich benutzt, um humanes Brustkrebsgewebe einzufärben. Dies wurde mit einem Leica BondTM System gemacht (Protocol F). Da wurde das Gewebe vorbehandelt mit einer Demaskierung mit Citrate pH 6 unter Hitze für 20 Minuten. Der ab105502 wurde dann mit einer Verdünnung von (5µg/ml) benutzt. Wie auch für den anderen Antikörper, kann ich empfehlen, diese Angaben als Ausgang zu benutzen und die Verdünnung und Demaskierugn in Ihrem Labor zu optimisieren - speziell auch die Länge der Erhitzung im Demaskierungspuffer hängt stark vom Gewebe und dessen Fixierung ab.
Der ab22131 wurde nicht in paraffin Schnitten getestet, sondern auf einfachen PFA fixierten Schnitten. Das Protokoll war etwas speziell - Sie können die Details auf dem Datenblatt unter dem Bild nachlesen. Um diesen Antikörper auf den Paraffinschnitten zu testen, kann ich Ihnen empfehlen eine Verdünnungsreihe von 1/50, 1/100 und1/200 empfehlen, wie auch Hitze Antigendemaskierung mit Citrate pH6 oder EDTA pH 9 auszuprobieren.
Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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Question
Answer

Thank you for contacting Abcam yesterday.

I have talked to the lab and we will be able to get the blocking peptide for ab87597 added to our catalogue. The product should be added in a day or so, therefore you should be able to purchase it by the end of the week.

If there is anything else I can help you with, please let me know.

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