Anti-CaMKII alpha antibody (ab87597)
Key features and details
- Goat polyclonal to CaMKII alpha
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CaMKII alpha antibody
See all CaMKII alpha primary antibodies -
Description
Goat polyclonal to CaMKII alpha -
Host species
Goat -
Specificity
Expected to recognize both reported isoforms of CaMKII (NP_057065.2 and NP_741960.1). This antibody does cross-react with CAMKII beta protein -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Dog -
Immunogen
Peptide with sequence
C-PRTAQSEETRVWHR
, corresponding to internal sequence amino acids 445-458 of human CaMKII alpha protein (NP_057065.2; NP_741960.1). (Peptide available as ab126462.) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab87597 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 55, 65 kDa (predicted molecular weight: 55 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 55, 65 kDa (predicted molecular weight: 55 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts. - Information by UniProt
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Database links
- Entrez Gene: 815 Human
- Entrez Gene: 12322 Mouse
- Entrez Gene: 25400 Rat
- Omim: 114078 Human
- SwissProt: Q9UQM7 Human
- SwissProt: P11798 Mouse
- SwissProt: P11275 Rat
- Unigene: 725992 Human
see all -
Alternative names
- Alpha CaMKII antibody
- Calcium calmodulin dependent protein kinase II antibody
- Calcium/calmodulin dependent protein kinase II alpha B subunit antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (6)
ab87597 has been referenced in 6 publications.
- De La Rossa A et al. Paradoxical neuronal hyperexcitability in a mouse model of mitochondrial pyruvate import deficiency. Elife 11:N/A (2022). PubMed: 35188099
- Li Q et al. GPR50 Distribution in the Mouse Cortex and Hippocampus. Neurochem Res 45:2312-2323 (2020). PubMed: 32696324
- Moretto E et al. TSPAN5 Enriched Microdomains Provide a Platform for Dendritic Spine Maturation through Neuroligin-1 Clustering. Cell Rep 29:1130-1146.e8 (2019). PubMed: 31665629
- Fraize N et al. Differential changes in hippocampal CaMKII and GluA1 activity after memory training involving different levels of adaptive forgetting. Learn Mem 24:86-94 (2017). WB ; Rat . PubMed: 28096498
- Gan JO et al. Adolescent social isolation enhances the plasmalemmal density of NMDA NR1 subunits in dendritic spines of principal neurons in the basolateral amygdala of adult mice. Neuroscience 258:174-83 (2014). IHC-FoFr ; Mouse . PubMed: 24231734
- Zhong W et al. Serotonin 5-HT3 receptor-mediated vomiting occurs via the activation of Ca2+/CaMKII-dependent ERK1/2 signaling in the least shrew (Cryptotis parva). PLoS One 9:e104718 (2014). IHC-Fr . PubMed: 25121483