Recombinant
RabMAb

Recombinant Anti-CaMKII alpha antibody [EPR1828] - BSA and Azide free (ab239906)

Overview

  • Product name

    Anti-CaMKII alpha antibody [EPR1828] - BSA and Azide free
    See all CaMKII alpha primary antibodies
  • Description

    Rabbit monoclonal [EPR1828] to CaMKII alpha - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC (PFA fixed), WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CaMKII alpha aa 1-100 (N terminal). The exact sequence is proprietary.

  • General notes

    Ab239906 is the carrier-free version of ab92332. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239906 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
IHC (PFA fixed) Use at an assay dependent concentration. PubMed: 24866701
WB Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization

    Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha CaMKII antibody
    • Calcium calmodulin dependent protein kinase II antibody
    • Calcium/calmodulin dependent protein kinase II alpha B subunit antibody
    • Calcium/calmodulin dependent protein kinase type II alpha chain antibody
    • Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha antibody
    • Calcium/calmodulin-dependent protein kinase II alpha antibody
    • Calcium/calmodulin-dependent protein kinase II-alpha antibody
    • Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody
    • Calcium/calmodulin-dependent protein kinase type IIA antibody
    • CaM kinase II alpha chain antibody
    • CaM kinase II alpha subunit antibody
    • CaM kinase II subunit alpha antibody
    • CaMK II alpha subunit antibody
    • CaMK-II subunit alpha antibody
    • Camk2a antibody
    • CAMKA antibody
    • CaMKII antibody
    • CaMKIINalpha antibody
    • EC 2.7.11.17 antibody
    • KCC2A_HUMAN antibody
    • KIAA0968 antibody
    • MGC123320 antibody
    • MGC139375 antibody
    • MGC155201 antibody
    • mKIAA0968 antibody
    • PK2CDD antibody
    • PKCCD antibody
    • R74975 antibody
    • zgc:112538 antibody
    • zgc:123320 antibody
    see all

Images

  • Overlay histogram showing SH-SY5Y cells stained with ab92332 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92332, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92332).

References

ab239906 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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