Recombinant Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880)
![Western blot - Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880)](https://www.abcam.com/ps/products/124/ab124880/Images/ab124880-7-rabbit-monoclonal-epr1830-47-to-camkkii-pt286-western-blot--brain-tissue-rat.jpg "Western blot - Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1830] to CaMKII alpha (phospho T286)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-CaMKII alpha (phospho T286) antibody [EPR1830]
See all CaMKII alpha primary antibodies -
Description
Rabbit monoclonal [EPR1830] to CaMKII alpha (phospho T286) -
Host species
Rabbit -
Specificity
Due to the homology between the immunogen peptide and other CAMKII isoforms, ab124880 may cross react with CAMKII delta (KCC2D, 93% homology) and CAMKII gamma (KCC2G, 79% homology) -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat
Does not react with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC: mouse cerebellum, rat cerebellum. WB: untreated mouse brain tissue lysate, untreated rat brain tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.60 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1830 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124880 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/4000. Detects a band of approximately 45-70 kDa (predicted molecular weight: 54 kDa).
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| IHC-P |
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
|
WB
1/4000. Detects a band of approximately 45-70 kDa (predicted molecular weight: 54 kDa). |
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IHC-P
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts. - Information by UniProt
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Database links
- Entrez Gene: 12322 Mouse
- Entrez Gene: 25400 Rat
- SwissProt: P11798 Mouse
- SwissProt: P11275 Rat
- Unigene: 131530 Mouse
- Unigene: 107499 Rat
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Alternative names
- Alpha CaMKII antibody
- Calcium calmodulin dependent protein kinase II antibody
- Calcium/calmodulin dependent protein kinase II alpha B subunit antibody
see all
Images
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Western blot - Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880)All lanes : Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880) at 1/4000 dilution
Lane 1 : Rat brain tissue lysate (untreated membrane)
Lane 2 : Rat brain tissue lysate (Lambda phosphatase treated membrane)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 54 kDa
Observed band size: 50,54 kDa why is the actual band size different from the predicted?
Exposure time: 92 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
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![Western blot - Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880)](https://www.abcam.com/ps/products/124/ab124880/Images/ab124880-6-rabbit-monoclonal-epr1830-47-to-camkkii-pt286-western-blot-brain-tissue-mouse.jpg)
Western blot - Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880)All lanes : Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880) at 1/4000 dilution
Lane 1 : Mouse brain tissue lysate (untreated membrane)
Lane 2 : Mouse brain tissue lysate (Lambda phosphatase treated membrane)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Predicted band size: 54 kDa
Observed band size: 50,54 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880)](https://www.abcam.com/ps/products/124/ab124880/Images/ab124880-5-rabbit-monoclonal-epr1830-47-to-camkkii-pt286-immunohistochemistry-cerebellum-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880)Immunohistochemical analysis of Paraffin-embedded sections Rat Cerebellum tissue labelling CaMKKII (pT286) with ab124880 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)(Ready to use).Rat cerebellum without Lambda Protein Phosphatase treatment staining on Rat Cerebellum is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)(Ready to use).
Positive staining on rat cerebellum without Lambda Protein Phosphatase treatment (image A); No signal was detected when tissues were treated with Lambda Protein Phosphatase (image B).The section was incubated with ab124880 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880)](https://www.abcam.com/ps/products/124/ab124880/Images/ab124880-4-rabbit-monoclonal-epr1830-47-to-camkkii-pt286-immunohistochemistry-cerebellum-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880)Immunohistochemical analysis of Paraffin-embedded sections Mouse Cerebellum tissue labelling CaMKKII (pT286) with ab124880 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at Ready to use dilution.Mouse cerebellum without Lambda Protein Phosphatase treatment staining on Mouse Cerebellum is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (abab209101) at Ready to use dilution.
Positive staining on mouse cerebellum without Lambda Protein Phosphatase treatment (image A); No signal was detected when tissues were treated with Lambda Protein Phosphatase (image B).The section was incubated with ab124880 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. -
![OI-RD Scanning - Anti-CaMKII alpha + CaMKII beta (phospho T286) antibody [EPR1830] (ab124880)](https://www.abcam.com/ps/products/124/ab124880/Images/ab124880-138815-anti-camkii-alpha-camkii-beta-phospho-t286-antibody-epr1830.jpg)
OI-RD Scanning - Anti-CaMKII alpha + CaMKII beta (phospho T286) antibody [EPR1830] (ab124880) -
![Anti-CaMKII alpha + CaMKII beta (phospho T286) antibody [EPR1830] (ab124880)](https://www.abcam.com/ps/products/124/ab124880/Images/ab124880-3-benefits-of-recombinant-antibodies.png)
Anti-CaMKII alpha + CaMKII beta (phospho T286) antibody [EPR1830] (ab124880)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab124880 has been referenced in 9 publications.
- Gu Y et al. Electroacupuncture Attenuates CFA-Induced Inflammatory Pain by Regulating CaMKII. Neural Plast 2020:8861994 (2020). PubMed: 33488694
- Xie W et al. Antidepressant-like effects of the Guanxin Danshen formula via mediation of the CaMK II-CREB-BDNF signalling pathway in chronic unpredictable mild stress-induced depressive rats. Ann Transl Med 7:564 (2019). PubMed: 31807545
- Zhu A et al. Wnt5a mediates chronic post-thoracotomy pain by regulating non-canonical pathways, nerve regeneration, and inflammation in rats. Cell Signal 44:51-61 (2018). WB . PubMed: 29339085
- Zhang L et al. Curcumin induces endoplasmic reticulum stress-associated apoptosis in human papillary thyroid carcinoma BCPAP cells via disruption of intracellular calcium homeostasis. Medicine (Baltimore) 97:e11095 (2018). PubMed: 29901626
- Zhang F et al. cTAGE5/MEA6 plays a critical role in neuronal cellular components trafficking and brain development. Proc Natl Acad Sci U S A 115:E9449-E9458 (2018). PubMed: 30224460
- Ahmed ME et al. Beneficial Effects of a CaMKIIa Inhibitor TatCN21 Peptide in Global Cerebral Ischemia. J Mol Neurosci 61:42-51 (2017). PubMed: 27604243
- Yanpallewar S et al. Nedd4-2 haploinsufficiency causes hyperactivity and increased sensitivity to inflammatory stimuli. Sci Rep 6:32957 (2016). WB . PubMed: 27604420
- La Montanara P et al. Synaptic synthesis, dephosphorylation, and degradation: a novel paradigm for an activity-dependent neuronal control of CDKL5. J Biol Chem 290:4512-27 (2015). PubMed: 25555910
- Haolong C et al. Enterovirus 71 VP1 activates calmodulin-dependent protein kinase II and results in the rearrangement of vimentin in human astrocyte cells. PLoS One 8:e73900 (2013). WB ; Human . PubMed: 24073199