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    camkii-alpha-phospho-t286-antibody-epr1830-ab124880.pdf

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Neuroscience Neurotransmission Calcium Signaling Calmodulin / CaMK
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RecombinantRabMAb

Recombinant Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880)

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Western blot - Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880)
  • Western blot - Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880)
  • OI-RD Scanning - Anti-CaMKII alpha + CaMKII beta (phospho T286) antibody [EPR1830] (ab124880)
  • Anti-CaMKII alpha + CaMKII beta (phospho T286) antibody [EPR1830] (ab124880)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1830] to CaMKII alpha (phospho T286)
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat

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Overview

  • Product name

    Anti-CaMKII alpha (phospho T286) antibody [EPR1830]
    See all CaMKII alpha primary antibodies
  • Description

    Rabbit monoclonal [EPR1830] to CaMKII alpha (phospho T286)
  • Host species

    Rabbit
  • Specificity

    Due to the homology between the immunogen peptide and other CAMKII isoforms, ab124880 may cross react with CAMKII delta (KCC2D, 93% homology) and CAMKII gamma (KCC2G, 79% homology)
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat
    Does not react with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC: mouse cerebellum, rat cerebellum. WB: untreated mouse brain tissue lysate, untreated rat brain tissue lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 3.60 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR1830
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • Calmodulin / CaMK
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calmodulin Pathway
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Other
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Types of disease
    • Cancer
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-CaMKII alpha + CaMKII beta (phospho T286) antibody [EPR1830] - BSA and Azide free (ab248035)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/4000. Detects a band of approximately 45-70 kDa (predicted molecular weight: 54 kDa).
IHC-P
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/4000. Detects a band of approximately 45-70 kDa (predicted molecular weight: 54 kDa).
IHC-P
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for ICC/IF or IP.

Target

  • Function

    CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization

    Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts.
  • Target information above from: UniProt accession Q9UQM7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 12322 Mouse
    • Entrez Gene: 25400 Rat
    • SwissProt: P11798 Mouse
    • SwissProt: P11275 Rat
    • Unigene: 131530 Mouse
    • Unigene: 107499 Rat
    • Alternative names

      • Alpha CaMKII antibody
      • Calcium calmodulin dependent protein kinase II antibody
      • Calcium/calmodulin dependent protein kinase II alpha B subunit antibody
      • Calcium/calmodulin dependent protein kinase type II alpha chain antibody
      • Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha antibody
      • Calcium/calmodulin-dependent protein kinase II alpha antibody
      • Calcium/calmodulin-dependent protein kinase II-alpha antibody
      • Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody
      • Calcium/calmodulin-dependent protein kinase type IIA antibody
      • CaM kinase II alpha chain antibody
      • CaM kinase II alpha subunit antibody
      • CaM kinase II subunit alpha antibody
      • CaMK II alpha subunit antibody
      • CaMK-II subunit alpha antibody
      • Camk2a antibody
      • CAMKA antibody
      • CaMKII antibody
      • CaMKIINalpha antibody
      • EC 2.7.11.17 antibody
      • KCC2A_HUMAN antibody
      • KIAA0968 antibody
      • MGC123320 antibody
      • MGC139375 antibody
      • MGC155201 antibody
      • mKIAA0968 antibody
      • PK2CDD antibody
      • PKCCD antibody
      • R74975 antibody
      • zgc:112538 antibody
      • zgc:123320 antibody
      see all

    Images

    • Western blot - Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880)
      Western blot - Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880)
      All lanes : Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880) at 1/4000 dilution

      Lane 1 : Rat brain tissue lysate (untreated membrane)
      Lane 2 : Rat brain tissue lysate (Lambda phosphatase treated membrane)

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 54 kDa
      Observed band size: 50,54 kDa why is the actual band size different from the predicted?


      Exposure time: 92 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST

    • Western blot - Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880)
      Western blot - Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880)
      All lanes : Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880) at 1/4000 dilution

      Lane 1 : Mouse brain tissue lysate (untreated membrane)
      Lane 2 : Mouse brain tissue lysate (Lambda phosphatase treated membrane)

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

      Predicted band size: 54 kDa
      Observed band size: 50,54 kDa why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880)

      Immunohistochemical analysis of Paraffin-embedded sections Rat Cerebellum tissue labelling CaMKKII (pT286) with ab124880 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)(Ready to use).Rat cerebellum without Lambda Protein Phosphatase treatment staining on Rat Cerebellum is observed. Counterstained with Hematoxylin.


      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)(Ready to use).
      Positive staining on rat cerebellum without Lambda Protein Phosphatase treatment (image A); No signal was detected when tissues were treated with Lambda Protein Phosphatase (image B).

      The section was incubated with ab124880 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880)

      Immunohistochemical analysis of Paraffin-embedded sections Mouse Cerebellum tissue labelling CaMKKII (pT286) with ab124880 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at Ready to use dilution.Mouse cerebellum without Lambda Protein Phosphatase treatment staining on Mouse Cerebellum is observed. Counterstained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (abab209101) at Ready to use dilution.
      Positive staining on mouse cerebellum without Lambda Protein Phosphatase treatment (image A); No signal was detected when tissues were treated with Lambda Protein Phosphatase (image B).

      The section was incubated with ab124880 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

    • OI-RD Scanning - Anti-CaMKII alpha + CaMKII beta (phospho T286) antibody [EPR1830] (ab124880)
      OI-RD Scanning - Anti-CaMKII alpha + CaMKII beta (phospho T286) antibody [EPR1830] (ab124880)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-CaMKII alpha + CaMKII beta (phospho T286) antibody [EPR1830] (ab124880)
      Anti-CaMKII alpha + CaMKII beta (phospho T286) antibody [EPR1830] (ab124880)

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (9)

    Publishing research using ab124880? Please let us know so that we can cite the reference in this datasheet.

    ab124880 has been referenced in 9 publications.

    • Gu Y  et al. Electroacupuncture Attenuates CFA-Induced Inflammatory Pain by Regulating CaMKII. Neural Plast 2020:8861994 (2020). PubMed: 33488694
    • Xie W  et al. Antidepressant-like effects of the Guanxin Danshen formula via mediation of the CaMK II-CREB-BDNF signalling pathway in chronic unpredictable mild stress-induced depressive rats. Ann Transl Med 7:564 (2019). PubMed: 31807545
    • Zhu A  et al. Wnt5a mediates chronic post-thoracotomy pain by regulating non-canonical pathways, nerve regeneration, and inflammation in rats. Cell Signal 44:51-61 (2018). WB . PubMed: 29339085
    • Zhang L  et al. Curcumin induces endoplasmic reticulum stress-associated apoptosis in human papillary thyroid carcinoma BCPAP cells via disruption of intracellular calcium homeostasis. Medicine (Baltimore) 97:e11095 (2018). PubMed: 29901626
    • Zhang F  et al. cTAGE5/MEA6 plays a critical role in neuronal cellular components trafficking and brain development. Proc Natl Acad Sci U S A 115:E9449-E9458 (2018). PubMed: 30224460
    • Ahmed ME  et al. Beneficial Effects of a CaMKIIa Inhibitor TatCN21 Peptide in Global Cerebral Ischemia. J Mol Neurosci 61:42-51 (2017). PubMed: 27604243
    • Yanpallewar S  et al. Nedd4-2 haploinsufficiency causes hyperactivity and increased sensitivity to inflammatory stimuli. Sci Rep 6:32957 (2016). WB . PubMed: 27604420
    • La Montanara P  et al. Synaptic synthesis, dephosphorylation, and degradation: a novel paradigm for an activity-dependent neuronal control of CDKL5. J Biol Chem 290:4512-27 (2015). PubMed: 25555910
    • Haolong C  et al. Enterovirus 71 VP1 activates calmodulin-dependent protein kinase II and results in the rearrangement of vimentin in human astrocyte cells. PLoS One 8:e73900 (2013). WB ; Human . PubMed: 24073199

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