Product nameAnti-CaMKII antibody [6G9]
See all CaMKII primary antibodies
DescriptionMouse monoclonal [6G9] to CaMKII
SpecificityThis antibody detects the CaMKII alpha subunit in both phosphorylated and non phosphorylated forms. Expected reactivity also includes the delta and gamma isoforms but not the beta isoform.
Tested applicationsSuitable for: IHC-Fr, IP, IHC-P, ICC/IF, WB, RIA, ELISAmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
Partially purified full length native rat protein.
- WB: Human, rat and mouse whole brain tissue lysates. Flow Cyt: PC12 cells. IHC - P: Rat normal brain FFPE tissue sections.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
Our Abpromise guarantee covers the use of ab22609 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 22201125|
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).|
|RIA||Use a concentration of 3 - 5 µg/ml.|
|ELISA||Use at an assay dependent concentration.|
FunctionCaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.
Cellular localizationCell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts.
- Information by UniProt
- Calcium/calmodulin dependent protein kinase II alpha antibody
- Calcium/calmodulin dependent protein kinase II beta antibody
- Calcium/calmodulin dependent protein kinase II delta antibody
All lanes : Anti-CaMKII antibody [6G9] (ab22609) at 10 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Human brain tissue lysate - total protein (ab29466)
Lysates/proteins at 20 µg per lane.
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 65 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
IHC image of ab22609 staining in Rat normal brain (Purkinje cells, cerebellum) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22609, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
This product has been referenced in:
- Wang Q et al. miR-223-3p/TIAL1 interaction is involved in the mechanisms associated with the neuroprotective effects of dexmedetomidine on hippocampal neuronal cells in vitro. Mol Med Rep 19:805-812 (2019). Read more (PubMed: 30569136) »
- Qi X et al. The Glutamatergic Postrhinal Cortex-Ventrolateral Orbitofrontal Cortex Pathway Regulates Spatial Memory Retrieval. Neurosci Bull N/A:N/A (2019). Read more (PubMed: 30604280) »