Overview

  • Product name

    Anti-CaMKII antibody [6G9] (Alexa Fluor® 488)
    See all CaMKII primary antibodies
  • Description

    Mouse monoclonal [6G9] to CaMKII (Alexa Fluor® 488)
  • Host species

    Mouse
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IF, IHC-Frmore details
  • Species reactivity

    Reacts with: Rat
  • Immunogen

    Full length native protein (purified) corresponding to Rat CaMKII. Partially purified full length native rat protein.

  • Positive control

    • IHC-Fr: rat frozen dentate gyrus tissue section ICC/IF: PC12
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

Our Abpromise guarantee covers the use of ab197497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
IHC-Fr 1/100.

This product gave a positive signal in rat frozen dentate gyrus tissue fixed with 10% formaldehyde (10 min).

Target

  • Function

    CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization

    Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts.
  • Information by UniProt
  • Database links

  • Alternative names

    • Calcium/calmodulin dependent protein kinase II alpha antibody
    • Calcium/calmodulin dependent protein kinase II beta antibody
    • Calcium/calmodulin dependent protein kinase II delta antibody
    • Calcium/calmodulin dependent protein kinase II gamma antibody
    • Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody
    • CaM kinase II alpha antibody
    • CaM kinase II antibody
    • CaM kinase II beta antibody
    • CaM kinase II delta antibody
    • CaM kinase II gamma antibody
    • CaM kinase II subunit alpha antibody
    • CaMK-II subunit alpha antibody
    • CAMK2 antibody
    • Camk2a antibody
    • CAMK2B antibody
    • CAMK2D antibody
    • CAMK2G antibody
    • CAMKA antibody
    • KCC2A_HUMAN antibody
    see all

Images

  • IHC image of CaMKII staining in a section of frozen normal rat dentate gyrus.

    The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab197497 at 1/100 (shown in green) and counterstained using ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

  • ab197497 staining CaMKII in PC12 cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab197497 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab197497 has not yet been referenced specifically in any publications.

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