Key features and details
- Mouse monoclonal [6G9] to CaMKII - BSA and Azide free
- Suitable for: WB, IP, ELISA, IHC-P, RIA, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Product nameAnti-CaMKII antibody [6G9] - BSA and Azide free
See all CaMKII primary antibodies
DescriptionMouse monoclonal [6G9] to CaMKII - BSA and Azide free
Tested applicationsSuitable for: WB, IP, ELISA, IHC-P, RIA, IHC-Frmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
Full length native protein (purified) corresponding to Rat CaMKII.
- IHC-P: Rat brain tissue.
Ab187488 is a PBS only version of ab22609.
We can conjugate this antibody to FITC for you.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferConstituent: PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab187488 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).|
|IP||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|RIA||Use a concentration of 3 - 5 µg/ml.|
|IHC-Fr||Use at an assay dependent concentration.|
FunctionCaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.
Cellular localizationCell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts.
- Information by UniProt
- Calcium/calmodulin dependent protein kinase II alpha antibody
- Calcium/calmodulin dependent protein kinase II beta antibody
- Calcium/calmodulin dependent protein kinase II delta antibody
IHC image of ab22609 staining in Rat normal brain (Purkinje cells, cerebellum) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22609, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab30).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab187488 has not yet been referenced specifically in any publications.