Overview

  • Product name

    Anti-CaMKII antibody [6G9] (HRP)
    See all CaMKII primary antibodies
  • Description

    Mouse monoclonal [6G9] to CaMKII (HRP)
  • Host species

    Mouse
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Full length native protein (purified) corresponding to Rat CaMKII.

  • Positive control

    • WB: Rat, mouse and human brain tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab197500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).

Target

Images

  • All lanes : Anti-CaMKII antibody [6G9] (HRP) (ab197500) at 1/5000 dilution

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Human) Tissue Lysate - adult normal tissue

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 56 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197500 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab197500 has not yet been referenced specifically in any publications.

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