Product nameAnti-CaMKII antibody [EPR6685(B)]
See all CaMKII primary antibodies
DescriptionRabbit monoclonal [EPR6685(B)] to CaMKII
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
A synthetic peptide, corresponding to residues in Human CaMKII (UniProt Q13554).
- Human pancreas, fetal kidney, HeLa, Jurkat, SH SY5Y, BxPC3 and C6 lysates; Permeabilized C6 cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 2.60 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab126789 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 45-70 kDa (predicted molecular weight: 51-73 kDa).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.
Cellular localizationCell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts.
- Information by UniProt
- Calcium/calmodulin dependent protein kinase II alpha antibody
- Calcium/calmodulin dependent protein kinase II beta antibody
- Calcium/calmodulin dependent protein kinase II delta antibody
All lanes : Anti-CaMKII antibody [EPR6685(B)] (ab126789) at 1/1000 dilution
Lane 1 : Human pancreas lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : HeLa lysate
Lane 4 : Jurkat lysate
Lane 5 : SH SY5Y lysate
Lane 6 : BxPC3 lysate
Lane 7 : C6 lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 51-73 kDa
ab126789 at 1/10 dilution staining CaMKII in permeabilized C6 cells by Flow cytometry (red) compared with a rabbit IgG negative control (green).
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab126789 has not yet been referenced specifically in any publications.