Overview

  • Product name

    Anti-CaMKII antibody [EPR6685(B)]
    See all CaMKII primary antibodies
  • Description

    Rabbit monoclonal [EPR6685(B)] to CaMKII
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide, corresponding to residues in Human CaMKII (UniProt Q13554).

  • Positive control

    • Human pancreas, fetal kidney, HeLa, Jurkat, SH SY5Y, BxPC3 and C6 lysates; Permeabilized C6 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 45-70 kDa (predicted molecular weight: 51-73 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    Images

    • All lanes : Anti-CaMKII antibody [EPR6685(B)] (ab126789) at 1/1000 dilution

      Lane 1 : Human pancreas lysate
      Lane 2 : Human fetal kidney lysate
      Lane 3 : HeLa lysate
      Lane 4 : Jurkat lysate
      Lane 5 : SH SY5Y lysate
      Lane 6 : BxPC3 lysate
      Lane 7 : C6 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 51-73 kDa

    • ab126789 at 1/10 dilution staining CaMKII in permeabilized C6 cells by Flow cytometry (red) compared with a rabbit IgG negative control (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab126789 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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