Recombinant
RabMAb

Recombinant Anti-CaMKII antibody [EPR6686(2)] (Allophycocyanin) (ab225178)

Overview

  • Product name

    Anti-CaMKII antibody [EPR6686(2)] (Allophycocyanin)
    See all CaMKII primary antibodies
  • Description

    Rabbit monoclonal [EPR6686(2)] to CaMKII (Allophycocyanin)
  • Host species

    Rabbit
  • Conjugation

    Allophycocyanin. Ex: 645nm, Em: 660nm
  • Specificity

    The immunogen shares 100% homology with CaMK2 beta, 93% homology with CaMK2 gamma and 73% homology with CaMK2 alpha and delta.
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity


    Predicted to work with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human CaMKII aa 550-650 (internal sequence).
    Database link: Q9UQM7

  • Positive control

    • Flow Cyt: U87MG cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab225178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.

The cellular localisation of this product has been verified in ICC/IF.

Target

Images

  • Overlay histogram showing U87MG cells stained with ab225178 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225178, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

    This antibody gave a positive signal in U87MG cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

References

ab225178 has not yet been referenced specifically in any publications.

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