Product nameAnti-CaMKII delta antibody [EPR13095]
See all CaMKII delta primary antibodies
DescriptionRabbit monoclonal [EPR13095] to CaMKII delta
SpecificityThe mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Mouse, Rat, Human
- SW480, A431 and HeLa whole cell lysate (ab150035); Human skeletal muscle tissue; SH-SY5Y; Mouse heart; Rat spleen; Human cardiac; Human thyroid .
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab181052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
FunctionCaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release.
Tissue specificityExpressed in cardiac muscle and skeletal muscle. Isoform Delta 3, isoform Delta 2, isoform Delta 8 and isoform Delta 9 are expressed in cardiac muscle. Isoform Delta 11 is expressed in skeletal muscle.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- Calcium / calmodulin dependent protein kinase 2 delta antibody
- Calcium / calmodulin dependent protein kinase II delta antibody
- calcium/calmodulin-dependent protein kinase (CaM kinase) II delta antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid carcinoma tissue sections labeling CaMKII delta with Purified ab181052 at 1:100 dilution (1.82 µg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cardiac muscle tissue sections labeling CaMKII delta with Purified ab181052 at 1:100 dilution (1.82 µg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Lane 1 : Anti-CaMKII delta antibody [EPR13095] (ab181052) at 1/1000 dilution
Lanes 2-4 : Anti-CaMKII delta antibody [EPR13095] (ab181052) at 1/1000 dilution (purified)
Lane 1 : HeLa ( Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Lane 2 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 20 µg
Lane 3 : Mouse heart lysates at 20 µg
Lane 4 : Rat spleen lysates at 20 µg
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 56 kDa
Blocking and diluting buffer : 5% NFDM/TBST
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: CaMKII delta knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: A431 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab181052 observed at 56 kDa. Red - loading control, ab8245, observed at 37 kDa.
Unpurified ab181052 was shown to recognize CaMKII delta when CaMKII delta knockout samples were used, along with additional cross-reactive bands. Wild-type and CaMKII delta knockout samples were subjected to SDS-PAGE. ab181052 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
All lanes : Anti-CaMKII delta antibody [EPR13095] (ab181052) at 1/2000 dilution (unpurified)
Lane 1 : SW480 whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Blocking/ Dilution buffer: 5% NFDM /TBST.
Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue sections labeling CaMKII delta using unpurified ab181052 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
This product has been referenced in:
- Huang T et al. Targeting the Ca2+/Calmodulin-dependent protein kinase II by Tetrandrine in human liver cancer cells. Biochem Biophys Res Commun 508:1227-1232 (2019). Read more (PubMed: 30554655) »
- Shi C et al. Leukocyte integrin signaling regulates FOXP1 gene expression via FOXP1-IT1 long non-coding RNA-mediated IRAK1 pathway. Biochim Biophys Acta Gene Regul Mech 1862:493-508 (2019). Read more (PubMed: 30831269) »