Recombinant Anti-CaMKII delta antibody [EPR13095] (ab181052)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13095] to CaMKII delta
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CaMKII delta antibody [EPR13095]
See all CaMKII delta primary antibodies -
Description
Rabbit monoclonal [EPR13095] to CaMKII delta -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T, HAP1, SW480, A431 and HeLa whole cell lysate (ab150035); Mouse heart tissue lysate; Rat spleen tissue lysate. IHC-P: Human thyroid carcinoma, cardiac and skeletal muscle tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13095 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
Notes |
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WB
1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
Target
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Function
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. -
Tissue specificity
Expressed in cardiac muscle and skeletal muscle. Isoform Delta 3, isoform Delta 2, isoform Delta 8 and isoform Delta 9 are expressed in cardiac muscle. Isoform Delta 11 is expressed in skeletal muscle. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 817 Human
- Entrez Gene: 108058 Mouse
- Entrez Gene: 24246 Rat
- Omim: 607708 Human
- SwissProt: Q13557 Human
- SwissProt: Q6PHZ2 Mouse
- SwissProt: P15791 Rat
- Unigene: 144114 Human
see all -
Alternative names
- Calcium / calmodulin dependent protein kinase 2 delta antibody
- Calcium / calmodulin dependent protein kinase II delta antibody
- calcium/calmodulin-dependent protein kinase (CaM kinase) II delta antibody
see all
Images
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All lanes : Anti-CaMKII delta antibody [EPR13095] (ab181052) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : CAMK2D knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Lanes 1- 2: Merged signal (red and green). Green - ab181052 observed at 50 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab181052 was shown to react with CaM-kinase II in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab267322 (knockout cell lysate ab257376) was used. Wild-type HEK-293T and CAMK2D knockout HEK-293T cell lysates were subjected to SDS-PAGE. ab181052 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] (ab181052)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid carcinoma tissue sections labeling CaMKII delta with Purified ab181052 at 1:100 dilution (1.82 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: CaMKII delta knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: A431 cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab181052 observed at 56 kDa. Red - loading control, ab8245, observed at 37 kDa.
Unpurified ab181052 was shown to recognize CaMKII delta when CaMKII delta knockout samples were used, along with additional cross-reactive bands. Wild-type and CaMKII delta knockout samples were subjected to SDS-PAGE. ab181052 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
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Lane 1 : Anti-CaMKII delta antibody [EPR13095] (ab181052) at 1/1000 dilution
Lanes 2-4 : Anti-CaMKII delta antibody [EPR13095] (ab181052) at 1/1000 dilution (purified)
Lane 1 : HeLa ( Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Lane 2 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 20 µg
Lane 3 : Mouse heart lysates at 20 µg
Lane 4 : Rat spleen lysates at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 56 kDaBlocking and diluting buffer : 5% NFDM/TBST
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All lanes : Anti-CaMKII delta antibody [EPR13095] (ab181052) at 1/2000 dilution (unpurified)
Lane 1 : SW480 whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDaBlocking/ Dilution buffer: 5% NFDM /TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] (ab181052)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cardiac muscle tissue sections labeling CaMKII delta with Purified ab181052 at 1:100 dilution (1.82 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] (ab181052)
Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue sections labeling CaMKII delta using unpurified ab181052 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Heat mediated antigen retrieval was performed citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (17)
ab181052 has been referenced in 17 publications.
- Wu Y et al. Tetrandrine attenuates left ventricular dysfunction in rats with myocardial infarction. Exp Ther Med 21:119 (2021). PubMed: 33335582
- Laudette M et al. Cyclic AMP-binding protein Epac1 acts as a metabolic sensor to promote cardiomyocyte lipotoxicity. Cell Death Dis 12:824 (2021). PubMed: 34471096
- Huangfu L et al. MicroRNA-135b/CAMK2D Axis Contribute to Malignant Progression of Gastric Cancer through EMT Process Remodeling. Int J Biol Sci 17:1940-1952 (2021). PubMed: 34131397
- Guo Y et al. The Ca2+-activated cation channel TRPM4 is a positive regulator of pressure overload-induced cardiac hypertrophy. Elife 10:N/A (2021). PubMed: 34190686
- Morgenstern J et al. Phosphorylation of T107 by CamKIId Regulates the Detoxification Efficiency and Proteomic Integrity of Glyoxalase 1. Cell Rep 32:108160 (2020). PubMed: 32966793