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    camkii-delta-antibody-epr13095-ab181052.pdf

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Neuroscience Neurotransmission Calcium Signaling Calmodulin / CaMK
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-CaMKII delta antibody [EPR13095] (ab181052)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (1) Submit a question References (17)

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Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] (ab181052)
  • Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052)
  • Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052)
  • Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] (ab181052)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] (ab181052)
  • Anti-CaMKII delta antibody [EPR13095] (ab181052)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13095] to CaMKII delta
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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Knockout
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Human CAMK2D (CaMKII delta) knockout HEK-293T cell line (ab267322)

View more associated products

Overview

  • Product name

    Anti-CaMKII delta antibody [EPR13095]
    See all CaMKII delta primary antibodies
  • Description

    Rabbit monoclonal [EPR13095] to CaMKII delta
  • Host species

    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T, HAP1, SW480, A431 and HeLa whole cell lysate (ab150035); Mouse heart tissue lysate; Rat spleen tissue lysate. IHC-P: Human thyroid carcinoma, cardiac and skeletal muscle tissues.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13095
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • Calmodulin / CaMK
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calmodulin Pathway
    • Cardiovascular
    • Heart
    • Apoptosis
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-CaMKII delta antibody [EPR13095] - BSA and Azide free (ab191588)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human CAMK2D (CaMKII delta) knockout HEK-293T cell line (ab267322)
  • KO cell lysates

    • Human CAMK2D (CaMKII delta) knockout HEK-293T cell lysate (ab257376)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • SW480 whole cell lysate (ab3957)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant human CaMKII delta protein (ab84552)
  • Related Products

    • Recombinant human CaMKII delta protein (ab84552)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Notes
WB
1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Application notes
Is unsuitable for ICC/IF.

Target

  • Function

    CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release.
  • Tissue specificity

    Expressed in cardiac muscle and skeletal muscle. Isoform Delta 3, isoform Delta 2, isoform Delta 8 and isoform Delta 9 are expressed in cardiac muscle. Isoform Delta 11 is expressed in skeletal muscle.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • Target information above from: UniProt accession Q13557 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 817 Human
    • Entrez Gene: 108058 Mouse
    • Entrez Gene: 24246 Rat
    • Omim: 607708 Human
    • SwissProt: Q13557 Human
    • SwissProt: Q6PHZ2 Mouse
    • SwissProt: P15791 Rat
    • Unigene: 144114 Human
    • Unigene: 255822 Mouse
    • Unigene: 87208 Rat
    see all
  • Alternative names

    • Calcium / calmodulin dependent protein kinase 2 delta antibody
    • Calcium / calmodulin dependent protein kinase II delta antibody
    • calcium/calmodulin-dependent protein kinase (CaM kinase) II delta antibody
    • calcium/calmodulin-dependent protein kinase type II delta chain antibody
    • Calcium/calmodulin-dependent protein kinase type II subunit delta antibody
    • CAM kinase 2 delta antibody
    • CAM kinase II delta antibody
    • CaM kinase II delta subunit antibody
    • CaM kinase II subunit delta antibody
    • CaM-kinase II delta chain antibody
    • CAMK 2d antibody
    • CaMK-II delta subunit antibody
    • CaMK-II subunit delta antibody
    • CAMK2D antibody
    • CAMKD antibody
    • CAMKI antibody
    • KCC2D_HUMAN antibody
    • RATCAMKI antibody
    see all

Images

  • Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052)
    Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052)
    All lanes : Anti-CaMKII delta antibody [EPR13095] (ab181052) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : CAMK2D knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 56 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Lanes 1- 2: Merged signal (red and green). Green - ab181052 observed at 50 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab181052 was shown to react with CaM-kinase II  in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab267322 (knockout cell lysate ab257376) was used. Wild-type HEK-293T and CAMK2D knockout HEK-293T cell lysates were subjected to SDS-PAGE. ab181052 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] (ab181052)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] (ab181052)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid carcinoma tissue sections labeling CaMKII delta with Purified ab181052 at 1:100 dilution (1.82 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052)
    Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: CaMKII delta knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: A431 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab181052 observed at 56 kDa. Red - loading control, ab8245, observed at 37 kDa.

    Unpurified ab181052 was shown to recognize CaMKII delta when CaMKII delta knockout samples were used, along with additional cross-reactive bands. Wild-type and CaMKII delta knockout samples were subjected to SDS-PAGE. ab181052 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052)
    Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052)
    Lane 1 : Anti-CaMKII delta antibody [EPR13095] (ab181052) at 1/1000 dilution
    Lanes 2-4 : Anti-CaMKII delta antibody [EPR13095] (ab181052) at 1/1000 dilution (purified)

    Lane 1 : HeLa ( Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
    Lane 2 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 20 µg
    Lane 3 : Mouse heart lysates at 20 µg
    Lane 4 : Rat spleen lysates at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 56 kDa



    Blocking and diluting buffer : 5% NFDM/TBST

  • Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052)
    Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052)
    All lanes : Anti-CaMKII delta antibody [EPR13095] (ab181052) at 1/2000 dilution (unpurified)

    Lane 1 : SW480 whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] (ab181052)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] (ab181052)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cardiac muscle tissue sections labeling CaMKII delta with Purified ab181052 at 1:100 dilution (1.82 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] (ab181052)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] (ab181052)

    Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue sections labeling CaMKII delta using unpurified ab181052 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Heat mediated antigen retrieval was performed citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-CaMKII delta antibody [EPR13095] (ab181052)
    Anti-CaMKII delta antibody [EPR13095] (ab181052)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (17)

Publishing research using ab181052? Please let us know so that we can cite the reference in this datasheet.

ab181052 has been referenced in 17 publications.

  • Wu Y  et al. Tetrandrine attenuates left ventricular dysfunction in rats with myocardial infarction. Exp Ther Med 21:119 (2021). PubMed: 33335582
  • Laudette M  et al. Cyclic AMP-binding protein Epac1 acts as a metabolic sensor to promote cardiomyocyte lipotoxicity. Cell Death Dis 12:824 (2021). PubMed: 34471096
  • Huangfu L  et al. MicroRNA-135b/CAMK2D Axis Contribute to Malignant Progression of Gastric Cancer through EMT Process Remodeling. Int J Biol Sci 17:1940-1952 (2021). PubMed: 34131397
  • Guo Y  et al. The Ca2+-activated cation channel TRPM4 is a positive regulator of pressure overload-induced cardiac hypertrophy. Elife 10:N/A (2021). PubMed: 34190686
  • Morgenstern J  et al. Phosphorylation of T107 by CamKIId Regulates the Detoxification Efficiency and Proteomic Integrity of Glyoxalase 1. Cell Rep 32:108160 (2020). PubMed: 32966793
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Western blot abreview for Anti-CaMKII delta antibody [EPR13095]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse Renal - Including KO)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
Mouse Renal - Including KO
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Edward Humphries

Verified customer

Submitted May 24 2017

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