Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release.
Expressed in cardiac muscle and skeletal muscle. Isoform Delta 3, isoform Delta 2, isoform Delta 8 and isoform Delta 9 are expressed in cardiac muscle. Isoform Delta 11 is expressed in skeletal muscle.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain.
Calcium / calmodulin dependent protein kinase 2 delta antibody
Calcium / calmodulin dependent protein kinase II delta antibody
calcium/calmodulin-dependent protein kinase (CaM kinase) II delta antibody
calcium/calmodulin-dependent protein kinase type II delta chain antibody
Calcium/calmodulin-dependent protein kinase type II subunit delta antibody
CAM kinase 2 delta antibody
CAM kinase II delta antibody
CaM kinase II delta subunit antibody
CaM kinase II subunit delta antibody
CaM-kinase II delta chain antibody
CAMK 2d antibody
CaMK-II delta subunit antibody
CaMK-II subunit delta antibody
Western blot - Anti-CaMKII delta antibody [EPR13095] - BSA and Azide free (ab191588)
This WB data was generated using the same anti-CAKII delta antibody clone [EPR13095] in a different buffer formulation (cat# ab181052).
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: CaMKII delta knockout HAP1 cell lysate (20 µg) Lane 3: HeLa cell lysate (20 µg) Lane 4: A431 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab181052 observed at 56 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab181052 was shown to recognize CaMKII delta when CaMKII delta knockout samples were used, along with additional cross-reactive bands. Wild-type and CaMKII delta knockout samples were subjected to SDS-PAGE. ab181052 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
has not yet been referenced specifically in any publications.
Publishing research using ab191588? Please let us know so that we can cite the reference in this datasheet.
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