Overview

  • Product name

    Anti-CamKII gamma antibody [8G10C1]
    See all CamKII gamma primary antibodies
  • Description

    Mouse monoclonal [8G10C1] to CamKII gamma
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human CamKII gamma aa 322-481. Expressed in E. Coli.
    Sequence:

    NKKSDGGVK KRKSSSSVHL MPQSNNKNSL VSPAQEPAPL QTAMEPQTTV VHNATDGIKG STESCNTTTE DEDLKARSPE GRSSRDRTAP SAGMQPQPSL CSSAMRKQEI IKITEQLIEA INNGDFEAYT KICDPGLTSF EPEALGNLVE GMDFHKFYFE N


    Database link: Q13555-1

  • Positive control

    • Human prostate cancer and bladder cancer tissues; Jurkat cells; PC12, Jurkat, T47D and HepG2 cell lysates; Human recombinant CamKII gamma (aa322-481) protein; Human recombinant CamKII gamma (aa322-481)-hIgGFc transfected HEK293 cell lysate.
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR280299-5 are from Tissue Culture Supernatant

Properties

Applications

Our Abpromise guarantee covers the use of ab201966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/1000.
WB 1/500 - 1/2000. Predicted molecular weight: 63 kDa.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

Images

  • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue labeling CamKII gamma with ab201966 at 1/200 dilution followed by DAB staining.

  • Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling CamKII gamma with ab201966 at 1/200 dilution followed by DAB staining. 

  • Flow cytometric analysis of Jurkat cells labeling CamKII gamma with ab201966 at 1/200 dilution (green). Negative control (red).

  • All lanes : Anti-CamKII gamma antibody [8G10C1] (ab201966) at 1/500 dilution

    Lane 1 : PC12 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : T47D cell lysate
    Lane 4 : HepG2 cell lysate

    Predicted band size: 63 kDa

  • All lanes : Anti-CamKII gamma antibody [8G10C1] (ab201966) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : Human recombinant CamKII gamma (aa322-481)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 63 kDa

  • Anti-CamKII gamma antibody [8G10C1] (ab201966) at 1/500 dilution + Human recombinant CamKII gamma (aa322-481) protein

    Predicted band size: 63 kDa



    (Expected MW is 44 kDa)

References

ab201966 has not yet been referenced specifically in any publications.

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