Overview

  • Product name

    Anti-CamKII gamma antibody
    See all CamKII gamma primary antibodies
  • Description

    Rabbit polyclonal to CamKII gamma
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Monkey
  • Immunogen

    Synthetic peptide within Human CamKII gamma aa 309-338 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.

  • Positive control

    • SKBR3 cell lysate, mouse heart tissue lysate, human hepatocarcinoma This antibody gave a positive result when used in the following methanol fixed cell lines: SKNSH

Properties

Applications

Our Abpromise guarantee covers the use of ab37999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/100 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-P 1/50 - 1/100.

Target

Images

  • ICC/IF image of ab37999 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab37999 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-CamKII gamma antibody (ab37999) at 1/1000 dilution + mouse heart tissue lysate at 35 µg/ml

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 56 kDa
    Observed band size: 56 kDa
    Additional bands at: 88 kDa. We are unsure as to the identity of these extra bands.



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • ab37999 at 1/100 dilution staining CamKII in human hepatocarcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by AEC staining.

References

This product has been referenced in:

  • Tetenborg S  et al. Differential Distribution of Retinal Ca2+/Calmodulin-Dependent Kinase II (CaMKII) Isoforms Indicates CaMKII-ß and -d as Specific Elements of Electrical Synapses Made of Connexin36 (Cx36). Front Mol Neurosci 10:425 (2017). Mouse . Read more (PubMed: 29311815) »
  • Yu L  et al. Fluid shear stress induces osteoblast differentiation and arrests the cell cycle at the G0 phase via the ERK1/2 pathway. Mol Med Rep 16:8699-8708 (2017). Read more (PubMed: 28990082) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Monkey Tissue sections (brain)
Antigen retrieval step
None
Permeabilization
Yes - triton 1%
Specification
brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 20°C
Fixative
Formaldehyde

Mrs. Francoise Geffroy

Verified customer

Submitted Mar 07 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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