Overview

  • Product name
    Anti-CaMKII (phospho T286) antibody [22B1]
    See all CaMKII primary antibodies
  • Description
    Mouse monoclonal [22B1] to CaMKII (phospho T286)
  • Host species
    Mouse
  • Specificity
    ab171095 detects the phosphorylated alpha subunit CaMKII only. It does not detect the non-phosphorylated alpha subunit CaMKII or the ~60 kDa beta subunit in either phosphorylation state.
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, ELISA, WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
  • Immunogen

    Synthetic peptide corresponding to Rat CaMKII aa 281-294 (phospho T286).
    Sequence:

    HRQETVDCLKKFN


    Database link: P11275

  • Positive control
    • Neuro-2a, C6, HeLa or U251 cells, Rat brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab171095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
WB 1/2000. Predicted molecular weight: 54 kDa.
ICC/IF 1/50 - 1/100.
IP Use at an assay dependent concentration.

Target

Images

  • Lanes 1-2 : Anti-CaMKII (phospho T286) antibody [22B1] (ab171095) at 1/2000 dilution
    Lanes 3-4 : A non phosphorylated CaMKII antibody at 1/2000 dilution

    Lane 1 : Unphosphorylated rat brain homogenate
    Lanes 2 & 4 : Phosphorylated Rat brain homogenate
    Lane 3 : Unphosphorylated Rat brain homogenate

    Predicted band size: 54 kDa

  • Immunofluorescence analysis of U251 cells labeling phosphorylated CaMKII (green) with ab171095 at a 1/100 dilution. Nuclei are stained (blue).

  • Immunofluorescence analysis of Neuro-2 cells (left image) or cells stimulated with Lambda-phosphatase (right image) labeling phosphorylated CaMKII (green) with ab171095 at a 1/50 dilution. F-actin (red) and nuclei (blue).

  • Immunofluorescence analysis of HeLa cells labeling phosphorylated CaMKII (green) with ab171095 at a 1/50 dilution.  Nuclei are stained (blue).

  • Immunofluorescence analysis of C6 cells labeling phospohrylated CaMKII (green) with ab171095 at a 1/100 dilution. Nuclei are stained (blue).

References

This product has been referenced in:
  • John S  et al. Bacoside A Induces Tumor Cell Death in Human Glioblastoma Cell Lines through Catastrophic Macropinocytosis. Front Mol Neurosci 10:171 (2017). IHC ; Human . Read more (PubMed: 28663722) »
  • Pchitskaya E  et al. Stim2-Eb3 Association and Morphology of Dendritic Spines in Hippocampal Neurons. Sci Rep 7:17625 (2017). Read more (PubMed: 29247211) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Cardiomyocytes)
Permeabilization
Yes - Triton x-100, 0.03%
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 31 2018

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jan 31 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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