Anti-CaMKII (phospho T286) antibody [22B1] (ab171095)
Key features and details
- Mouse monoclonal [22B1] to CaMKII (phospho T286)
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-CaMKII (phospho T286) antibody [22B1]
See all CaMKII primary antibodies -
Description
Mouse monoclonal [22B1] to CaMKII (phospho T286) -
Host species
Mouse -
Specificity
ab171095 detects the phosphorylated alpha subunit CaMKII only. It does not detect the non-phosphorylated alpha subunit CaMKII or the ~60 kDa beta subunit in either phosphorylation state. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Rat CaMKII aa 281-294 (phospho T286).
Sequence:HRQETVDCLKKFN
Database link: P11275 -
Positive control
- Neuro-2a, C6, HeLa or U251 cells, Rat brain lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
22B1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/2000. Predicted molecular weight: 54 kDa.
|
ICC/IF |
1/100.
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Notes |
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WB
1/2000. Predicted molecular weight: 54 kDa. |
ICC/IF
1/100. |
Target
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Function
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts. - Information by UniProt
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Database links
- Entrez Gene: 815 Human
- Entrez Gene: 816 Human
- Entrez Gene: 817 Human
- Entrez Gene: 818 Human
- Entrez Gene: 108058 Mouse
- Entrez Gene: 12322 Mouse
- Entrez Gene: 12323 Mouse
- Entrez Gene: 12325 Mouse
see all -
Alternative names
- Calcium/calmodulin dependent protein kinase II alpha antibody
- Calcium/calmodulin dependent protein kinase II beta antibody
- Calcium/calmodulin dependent protein kinase II delta antibody
see all
Images
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Lanes 1-2 : Anti-CaMKII (phospho T286) antibody [22B1] (ab171095) at 1/2000 dilution
Lanes 3-4 : A non phosphorylated CaMKII antibody at 1/2000 dilution
Lane 1 : Unphosphorylated rat brain homogenate
Lanes 2 & 4 : Phosphorylated Rat brain homogenate
Lane 3 : Unphosphorylated Rat brain homogenate
Predicted band size: 54 kDa -
Immunofluorescence analysis of U251 (human) cells labeling phosphorylated CaMKII (green) with ab171095 at a 1/100 dilution. Nuclei are stained (blue).
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Immunofluorescence analysis of Neuro-2 (mouse) cells (left image) or cells stimulated with Lambda-phosphatase (right image) labeling phosphorylated CaMKII (green) with ab171095 at a 1/50 dilution. F-actin (red) and nuclei (blue).
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Immunofluorescence analysis of HeLa cells labeling phosphorylated CaMKII (green) with ab171095 at a 1/50 dilution. Nuclei are stained (blue).
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Immunofluorescence analysis of C6 cells labeling phospohrylated CaMKII (green) with ab171095 at a 1/100 dilution. Nuclei are stained (blue).
Protocols
Datasheets and documents
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Datasheet download
References (24)
ab171095 has been referenced in 24 publications.
- Yeh KC et al. Soybean Meal Extract Preserves Memory Ability by Increasing Presynaptic Function and Modulating Gut Microbiota in Rats. Mol Neurobiol 59:1649-1664 (2022). PubMed: 35001354
- Li ML et al. LY395756 promotes NR2B expression via activation of AKT/CREB signaling in the juvenile methylazoxymethanol mice model of schizophrenia. Brain Behav 12:e2466 (2022). PubMed: 35025141
- Han MX et al. Gao-Zi-Yao improves learning and memory function in old spontaneous hypertensive rats. BMC Complement Med Ther 22:147 (2022). PubMed: 35643519
- Zhou XH et al. Remimazolam induced cognitive dysfunction in mice via glutamate excitotoxicity. Transl Neurosci 13:104-115 (2022). PubMed: 35734308
- Zhu M et al. Calsyntenin-1 Promotes Doxorubicin-induced Dilated Cardiomyopathy in Rats. Cardiovasc Drugs Ther N/A:N/A (2022). PubMed: 36350487