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Neuroscience Neurotransmission Calcium Signaling Calmodulin / CaMK
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Anti-CaMKII (phospho T286) antibody (ab32678)

  • Datasheet
  • SDS
Reviews (26)Q&A (5)References (52)

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Western blot - Anti-CaMKII (phospho T286) antibody (ab32678)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII (phospho T286) antibody (ab32678)
  • Western blot - Anti-CaMKII (phospho T286) antibody (ab32678)
  • Western blot - Anti-CaMKII (phospho T286) antibody (ab32678)

Key features and details

  • Rabbit polyclonal to CaMKII (phospho T286)
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-CaMKII antibody [EPR6686(2)] (ab134041)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-CaMKII (phospho T286) antibody
    See all CaMKII primary antibodies
  • Description

    Rabbit polyclonal to CaMKII (phospho T286)
  • Host species

    Rabbit
  • Specificity

    This antibody is specific for the ~50 kDa alpha CaM Kinase II subunit and the ~60 kDa beta CaM Kinase II subunit phosphorylated at Thr286 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Rat
    See all applications and species data
  • Immunogen

    Synthetic phosphopeptide corresponding to amino acids surrounding phospho-286 from Rat brain CaMKII.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.50
    Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • Calmodulin / CaMK
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • Myricetin, irreversible TrxR inhibitor (ab120721)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Rat
All applications
Chicken
Cow
Application Abreviews Notes
WB (16)
1/1000. Predicted molecular weight: 50 kDa. Predicted molecular weight: ~50 kDa for the alpha subunit and ~60 kDa for the beta subunit of CaMKII.
IHC-P (1)
Use a concentration of 2 µg/ml.
Notes
WB
1/1000. Predicted molecular weight: 50 kDa. Predicted molecular weight: ~50 kDa for the alpha subunit and ~60 kDa for the beta subunit of CaMKII.
IHC-P
Use a concentration of 2 µg/ml.

Target

  • Function

    CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization

    Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts.
  • Target information above from: UniProt accession Q9UQM7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 374174 Chicken
    • Entrez Gene: 374175 Chicken
    • Entrez Gene: 422688 Chicken
    • Entrez Gene: 423737 Chicken
    • Entrez Gene: 282162 Cow
    • Entrez Gene: 525416 Cow
    • Entrez Gene: 530719 Cow
    • Entrez Gene: 532713 Cow
    • Entrez Gene: 815 Human
    • Entrez Gene: 816 Human
    • Entrez Gene: 817 Human
    • Entrez Gene: 818 Human
    • Entrez Gene: 171140 Rat
    • Entrez Gene: 24245 Rat
    • Entrez Gene: 24246 Rat
    • Entrez Gene: 25400 Rat
    • Omim: 114078 Human
    • SwissProt: Q5ZKI0 Chicken
    • SwissProt: Q2HJF7 Cow
    • SwissProt: Q3MHJ9 Cow
    • SwissProt: Q13554 Human
    • SwissProt: Q13555 Human
    • SwissProt: Q13557 Human
    • SwissProt: Q9UQM7 Human
    • SwissProt: P08413 Rat
    • SwissProt: P11275 Rat
    • SwissProt: P11730 Rat
    • SwissProt: P15791 Rat
    • Unigene: 725992 Human
    • Unigene: 107499 Rat
    see all
  • Alternative names

    • Calcium/calmodulin dependent protein kinase II alpha antibody
    • Calcium/calmodulin dependent protein kinase II beta antibody
    • Calcium/calmodulin dependent protein kinase II delta antibody
    • Calcium/calmodulin dependent protein kinase II gamma antibody
    • Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody
    • CaM kinase II alpha antibody
    • CaM kinase II antibody
    • CaM kinase II beta antibody
    • CaM kinase II delta antibody
    • CaM kinase II gamma antibody
    • CaM kinase II subunit alpha antibody
    • CaMK-II subunit alpha antibody
    • CAMK2 antibody
    • Camk2a antibody
    • CAMK2B antibody
    • CAMK2D antibody
    • CAMK2G antibody
    • CAMKA antibody
    • KCC2A_HUMAN antibody
    see all

Images

  • Western blot - Anti-CaMKII (phospho T286) antibody (ab32678)
    Western blot - Anti-CaMKII (phospho T286) antibody (ab32678)This image is courtesy of an anonymous Abreview.
    All lanes : Anti-CaMKII (phospho T286) antibody (ab32678) at 1/1000 dilution

    Lane 1 : Human myocardium tissue lysate, heart failure
    Lane 2 : Human myocardium tissue lysate, hypertrophy
    Lane 3 : Human myocardium tissue lysate, non-failing

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP conjugated sheep anti-rabbit at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 50 kDa


    Exposure time: 30 seconds


    Blocked with 5% milk for 1 hour at RT.

    Incubated with primary antibody for 14 hours at 4°C in TBS-T20.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII (phospho T286) antibody (ab32678)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII (phospho T286) antibody (ab32678)

    ab32678 (2µg/ml) staining CaMKII (phospho T286) in human Brain:cortex:frontal-lateral using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear/cytoplasmic compartments within the stellate cells and the myelinated fibres of white matter region .
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

  • Western blot - Anti-CaMKII (phospho T286) antibody (ab32678)
    Western blot - Anti-CaMKII (phospho T286) antibody (ab32678)

    PC 12 cells were incubated at 37°C for 30 minutes with vehicle control (0 µM) and different concentrations of myricetin (ab120721). Increased expression of CaMKII (phospho T286) (ab32678) in PC 12 cells correlates with an increase in myricetin concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab32678at 1/500 dilution and ab52476 at 1/500 dilution overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

  • Western blot - Anti-CaMKII (phospho T286) antibody (ab32678)
    Western blot - Anti-CaMKII (phospho T286) antibody (ab32678)
    All lanes : Anti-CaMKII (phospho T286) antibody (ab32678) at 1/1000 dilution

    Lane 1 : Rat brain cortex lysate.
    Lane 2 : Rat brain cortex lysate preincubated with lambda-phosphatase.

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 50 kDa
    Observed band size: 50,60 kDa
    why is the actual band size different from the predicted?



    Predicted molecular weight: ~50 kDa for the alpha subunit and ~60 kDa for the beta subunit of CaMKII.
    These images show the phosphospecificity of this antibody.

Protocols

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (52)

    Publishing research using ab32678? Please let us know so that we can cite the reference in this datasheet.

    ab32678 has been referenced in 52 publications.

    • Jiang S  et al. Astragaloside IV attenuates chronic intermittent hypoxia-induced myocardial injury by modulating Ca2+ homeostasis. Cell Biochem Funct N/A:N/A (2020). PubMed: 32306464
    • Kari S  et al. PKA Activates AMPK Through LKB1 Signaling in Follicular Thyroid Cancer. Front Endocrinol (Lausanne) 10:769 (2019). PubMed: 31798532
    • Xie W  et al. Antidepressant-like effects of the Guanxin Danshen formula via mediation of the CaMK II-CREB-BDNF signalling pathway in chronic unpredictable mild stress-induced depressive rats. Ann Transl Med 7:564 (2019). PubMed: 31807545
    • Eizuka K  et al. SYT12 plays a critical role in oral cancer and may be a novel therapeutic target. J Cancer 10:4913-4920 (2019). PubMed: 31598163
    • Qin R  et al. Exercise training reduces ventricular arrhythmias through restoring calcium handling and sympathetic tone in myocardial infarction mice. Physiol Rep 7:e13972 (2019). PubMed: 30806037
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 31 Abreviews or Q&A

    Western blot abreview for Anti-CaMKII (phospho T286) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (BV2 microglia)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Treatment
    5µM LPA for different time points
    Specification
    BV2 microglia
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Dr. Lisha Joshi

    Verified customer

    Submitted Jun 19 2019

    Western blot abreview for Anti-CaMKII (phospho T286) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (primary culture cortex cell)
    Gel Running Conditions
    Non-reduced Denaturing (15% SDS-PAGE)
    Loading amount
    15 µg
    Treatment
    RIPA & 1%Protease inhibitor for 15min on ice
    Specification
    primary culture cortex cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 08 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-CaMKII (phospho T286) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Pig Tissue sections (Left ventricle)
    Permeabilization
    No
    Specification
    Left ventricle
    Blocking step
    (agent) for 10 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Acetone
    Read More

    Christopher Trummer

    Verified customer

    Submitted Oct 02 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-CaMKII (phospho T286) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Left ventricle)
    Permeabilization
    No
    Specification
    Left ventricle
    Blocking step
    (agent) for 10 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Acetone
    Read More

    Christopher Trummer

    Verified customer

    Submitted Oct 02 2018

    Western blot abreview for Anti-CaMKII (phospho T286) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Cortex)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    50 µg
    Specification
    Cortex
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Christopher Trummer

    Verified customer

    Submitted Oct 02 2018

    Western blot abreview for Anti-CaMKII (phospho T286) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Guinea pig Tissue lysate - whole (Left ventricle)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    50 µg
    Specification
    Left ventricle
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Christopher Trummer

    Verified customer

    Submitted Oct 02 2018

    Western blot abreview for Anti-CaMKII (phospho T286) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Pig Tissue lysate - whole (Left ventricle)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    50 µg
    Specification
    Left ventricle
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Christopher Trummer

    Verified customer

    Submitted Oct 02 2018

    Western blot abreview for Anti-CaMKII (phospho T286) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Left ventricle)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    50 µg
    Specification
    Left ventricle
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Christopher Trummer

    Verified customer

    Submitted Oct 02 2018

    Western blot abreview for Anti-CaMKII (phospho T286) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rabbit Tissue lysate - whole (Cortex)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    Cortex
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Christopher Trummer

    Verified customer

    Submitted Oct 02 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-CaMKII (phospho T286) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Left ventricle)
    Permeabilization
    No
    Specification
    Left ventricle
    Blocking step
    (agent) for 10 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Acetone
    Read More

    Christopher Trummer

    Verified customer

    Submitted Oct 02 2018

    1-10 of 31 Abreviews or Q&A

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