Anti-CaMKII (phospho T286) antibody (ab32678)
Key features and details
- Rabbit polyclonal to CaMKII (phospho T286)
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
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Overview
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Product name
Anti-CaMKII (phospho T286) antibody
See all CaMKII primary antibodies -
Description
Rabbit polyclonal to CaMKII (phospho T286) -
Host species
Rabbit -
Specificity
This antibody is specific for the ~50 kDa alpha CaM Kinase II subunit and the ~60 kDa beta CaM Kinase II subunit phosphorylated at Thr286 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Chicken, Cow
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Immunogen
Synthetic peptide corresponding to Rat CaMKII (phospho T286).
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Positive control
- WB: Rat cortex lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (16) |
1/1000. Predicted molecular weight: 50 kDa. Predicted molecular weight: ~50 kDa for the alpha subunit and ~60 kDa for the beta subunit of CaMKII.
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| Notes |
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WB
1/1000. Predicted molecular weight: 50 kDa. Predicted molecular weight: ~50 kDa for the alpha subunit and ~60 kDa for the beta subunit of CaMKII. |
Target
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Function
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts. - Information by UniProt
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Database links
- Entrez Gene: 374174 Chicken
- Entrez Gene: 374175 Chicken
- Entrez Gene: 422688 Chicken
- Entrez Gene: 423737 Chicken
- Entrez Gene: 282162 Cow
- Entrez Gene: 525416 Cow
- Entrez Gene: 530719 Cow
- Entrez Gene: 532713 Cow
see all -
Alternative names
- Calcium/calmodulin dependent protein kinase II alpha antibody
- Calcium/calmodulin dependent protein kinase II beta antibody
- Calcium/calmodulin dependent protein kinase II delta antibody
see all
Images
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Western blot - Anti-CaMKII (phospho T286) antibody (ab32678)All lanes : Anti-CaMKII (phospho T286) antibody (ab32678) at 1/1000 dilution
Lane 1 : Rat brain cortex lysate.
Lane 2 : Rat brain cortex lysate preincubated with lambda-phosphatase.
Lysates/proteins at 50 µg per lane.
Predicted band size: 50 kDa
Observed band size: 50,60 kDa why is the actual band size different from the predicted?
Predicted molecular weight: ~50 kDa for the alpha subunit and ~60 kDa for the beta subunit of CaMKII.
These images show the phosphospecificity of this antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (64)
ab32678 has been referenced in 64 publications.
- Rivera DS et al. "Live together, die alone": The effect of re-socialization on behavioural performance and social-affective brain-related proteins after a long-term chronic social isolation stress. Neurobiol Stress 14:100289 (2021). PubMed: 33426200
- Wang W et al. Inhibition of adenosine kinase attenuates myocardial ischaemia/reperfusion injury. J Cell Mol Med 25:2931-2943 (2021). PubMed: 33523568
- Jiang S et al. Astragaloside IV attenuates chronic intermittent hypoxia-induced myocardial injury by modulating Ca2+ homeostasis. Cell Biochem Funct N/A:N/A (2020). PubMed: 32306464
- Cheng WL et al. Macrophage migration inhibitory factor increases atrial arrhythmogenesis through CD74 signaling. Transl Res 216:43-56 (2020). PubMed: 31669150
- Yang X et al. Enhanced cardiomyocyte reactive oxygen species signaling promotes ibrutinib-induced atrial fibrillation. Redox Biol 30:101432 (2020). PubMed: 31986467