Key features and details
- Rabbit polyclonal to CAMKIV
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CAMKIV antibody
See all CAMKIV primary antibodies
DescriptionRabbit polyclonal to CAMKIV
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human CAMKIV aa 304-473.
ALQHPWVTGKAANFVHMDTAQKKLQEFNARRKLKAAVKAVVASSRLGSAS SSHGSIQESHKASRDPSPIQDGNEDMKAIPEGEKIQGDGAQAAVKGAQAE LMKVQALEKVKGADINAEEAPKMVPKAVEDGIKVADLELEEGLAEEKLKT VEEAAAPREGQGSSAVGFEVPQQDVILPEY
Database link: Q16566
- WB: HEK-293T cell lysate. IP: Jurkat whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab235058 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/200 - 1/2000.|
|WB||1/1000 - 1/5000.|
FunctionCalcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory.
Tissue specificityExpressed in epithelial ovarian cancer tissue.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.
modificationsAutophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200.
Cellular localizationCytoplasm. Nucleus. Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix.
- Information by UniProt
- Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody
- Brain Ca(2+) calmodulin dependent protein kinase type IV antibody
- Brain Ca++-calmodulin dependent protein kinase type IV antibody
Anti-CAMKIV antibody (ab235058) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Goat polyclonal to rabbit IgG at 1/10000 dilution
CAMKIV was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab235058. Western blot was performed from the immunoprecipitate using ab235058 at 1/200 dilution. HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody at 1/50000 dilution.
Lane 1: 1 µg rabbit monoclonal IgG instead of ab235058 in Jurkat whole cell lysate.
Lane 2: ab235058 IP in Jurkat whole cell lysate.
Lane 3: Jurkat whole cell lysate 20 µg (Input).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235058 has not yet been referenced specifically in any publications.