Product nameAnti-CAMKIV antibody
See all CAMKIV primary antibodies
DescriptionRabbit polyclonal to CAMKIV
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Chicken, Cow, Dog, Xenopus laevis, Chimpanzee, Zebrafish
- Rat brain or Jurkat cells lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Purification notesThis antiserum is directed against human CaM Kinase IV protein. The product was delipidated, defibrinated followed by buffering and clarification.
Our Abpromise guarantee covers the use of ab34868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 52 kDa.|
|ELISA||1/5000 - 1/25000.|
|ICC/IF||Use at an assay dependent dilution.|
FunctionCalcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory.
Tissue specificityExpressed in epithelial ovarian cancer tissue.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.
modificationsAutophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200.
Cellular localizationCytoplasm. Nucleus. Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix.
- Information by UniProt
- Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody
- Brain Ca(2+) calmodulin dependent protein kinase type IV antibody
- Brain Ca++-calmodulin dependent protein kinase type IV antibody
All lanes : Anti-CAMKIV antibody (ab34868) at 1/1000 dilution (for 2 hours at room temperature)
Lane 1 : Rat brain lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Rat brain lysate with Immunizing peptide
Lane 4 : Jurkat cells lysate with Immunizing peptide
Lysates/proteins at 35 µg per lane.
All lanes : IRDye TM800 conjugated Gt-a-Rabbit IgG for 45 min at room temperature at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
ab34868 has not yet been referenced specifically in any publications.